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Home » Gene Synthesis & Molecular Biology Services » PKCμ Antibody, pAb, Rabbit

PKCμ Antibody, pAb, Rabbit

PKCμ Antibody, PAb, Rabbit

Western blot analysis of cell lysates using 2 µg/ml Rabbit Anti-PKCμ Polyclonal Antibody (GenScript, A00909)
Lane 1: Hela cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: NIH/3T3 cell lysate
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

PKCμ Antibody, PAb, Rabbit

ImmunohistochemistryanalysisofhumanKidney tissue slide (Paraffin embedded) using PKCμ Antibody, pAb, Rabbit (GenScript, Cat. No.A00909, 10 ug/ml).

PKCμ Antibody, PAb, Rabbit

ImmunohistochemistryanalysisofhumanBrain, Cortex tissue slide (Paraffin embedded) using PKCμ Antibody, pAb, Rabbit (GenScript, Cat. No.A00909, 10 ug/ml).

Synonyms Rabbit Anti-PKCμ pAb;
Description Protein kinase C (PKC, EC 2.7.11.13) is a cyclic, nucleotide-independent enzyme that phosphorylates serine and threonine residues. The PKC family is divided into three groups depending on each the enzymes cofactor requirements: conventional (c)PKC isoforms (α, βI, βII, and γ) require diacylglycerol (DAG), Ca2+, and phospholipids for activation; novel (n)PKC isoforms (δ, ε, η (also known as PKC-L), θ, and µ the mouse homolog of human PKCµ (also known as PKD)) require DAG but not Ca2+ for activation; and atypical (a)PKC isoforms, (ζ, ι, and λ, the mouse homolog of human PKC ι) require neither Ca2+ nor DAG. A new PKC member has recently been discovered and is referred to as PKCν.
PKCs phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. These kinases also serve as major receptors for phorbol esters, a class of tumor promoters. Every member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
PKCμ, also known as nPKC-D1 and nPKC-mu, is a member of PKC family of serine/threonine kinases. PKCμ has two characteristic cysteine-rich repeats, a conserved ATP-binding consensus sequence in the kinase domain, and the invariate aspartate essential for kinase activity.
GenScript Rabbit Anti-PKCμ Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide corresponding to residues of human PKCμ protein.
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide (KLH-coupled) corresponding to residues of human PKCμ protein.
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-PKCμ Polyclonal Antibody detects endogenous levels of human and mouse PKCμ protein. No cross-reactivity to other PKC isoforms is detected.
Species Reactivity Human, mouse
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2 µg/ml
Other applications: user-optimized
Immunohistochemistry:5-10 µg/ml

  • PKCμ Antibody, PAb, Rabbit
  • PKCμ Antibody, PAb, Rabbit

    Western blot analysis of cell lysates using 2 µg/ml Rabbit Anti-PKCμ Polyclonal Antibody (GenScript, A00909)
    Lane 1: Hela cell lysate
    Lane 2: HEK-293 cell lysate
    Lane 3: NIH/3T3 cell lysate
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

  • PKCμ Antibody, PAb, Rabbit
  • PKCμ Antibody, PAb, Rabbit

    ImmunohistochemistryanalysisofhumanKidney tissue slide (Paraffin embedded) using PKCμ Antibody, pAb, Rabbit (GenScript, Cat. No.A00909, 10 ug/ml).

  • PKCμ Antibody, PAb, Rabbit
  • PKCμ Antibody, PAb, Rabbit

    ImmunohistochemistryanalysisofhumanBrain, Cortex tissue slide (Paraffin embedded) using PKCμ Antibody, pAb, Rabbit (GenScript, Cat. No.A00909, 10 ug/ml).




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Cat. No. A00909
Size
Price
40 ug $99.00