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Catalog Products » Other Products » New Products » Estrogen Receptor Beta Antibody, pAb, Rabbit

Estrogen Receptor Beta Antibody, pAb, Rabbit

Synonyms Rabbit Anti-Estrogen Receptor Beta pAb;
Description The estrogen receptor (ESR) beta is a ligand-activated transcription factor. It is composed of several domains important for hormone binding, DNA binding, and activation of transcription. Estrogen receptor beta is highly homologous to estrogen receptor alpha, and it was initially cloned and characterized from testis.
GenScript Rabbit Anti-Estrogen Receptor Beta Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide, and purified from rabbit antiserum by immunoaffinity chromatography.
Host Species Rabbit
Antigen Species Mouse
Conjugation Unconjugated
Immunogen Synthetic peptide conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide
Specificity GenScript Rabbit Anti-Estrogen Receptor Beta Polyclonal Antibody reacts with mouse estrogen receptor beta.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.0125-0.1 μg/ml
Western blot: 1.0 μg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Estrogen Receptor Beta Antibody, PAb, Rabbit
Estrogen Receptor Beta Antibody, PAb, Rabbit

Western blot analysis:
Lane 1: EasyWestern Protein Standard (GenScript, MM0908)
Lane 2: Mouse brain tissue lysate

Primary antibody:
1 µg/ml Rabbit Anti-Estrogen Receptor Beta Polyclonal Antibody (GenScript, A00939)

Secondary antibody:
Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:6,000)




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Cat. No. A00939
Size
Price
40 ug $99.00
 
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