|Synonyms||Rabbit Anti-PGK1 pAb;|
|Description||The PGK1 gene encodes phosphoglycerate kinase-1, which catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and is proposed to participate in the angiogenic process as a disulfide reductase. Mutations in PGK1 may be associated with hemolytic anemia.
GenScript Rabbit Anti-PGK1 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide.
|Immunogen||Synthetic peptide conjugated to KLH|
|Concentration||0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide|
|Specificity||GenScript Rabbit Anti-PGK1 reacts with human PGK1. Sequence homology predicts that it will also react with mouse, chicken, fruit fly, and zebrafish PGK1.|
|Predicted Band Size||44 kD|
|Observed Band Size||44 kD|
|Storage||The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.03-0.125 µg/ml
Western blot: 1.0 µg/ml
Other applications: user-optimized
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)
|Cat. No. A00961|