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PGK1 Antibody, pAb, Rabbit

Synonyms Rabbit Anti-PGK1 pAb;
Description The PGK1 gene encodes phosphoglycerate kinase-1, which catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and is proposed to participate in the angiogenic process as a disulfide reductase. Mutations in PGK1 may be associated with hemolytic anemia.
GenScript Rabbit Anti-PGK1 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide.
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide
Specificity GenScript Rabbit Anti-PGK1 reacts with human PGK1. Sequence homology predicts that it will also react with mouse, chicken, fruit fly, and zebrafish PGK1.
Predicted Band Size 44 kD
Observed Band Size 44 kD
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.03-0.125 µg/ml
Western blot: 1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

PGK1 Antibody, PAb, Rabbit
PGK1 Antibody, PAb, Rabbit

Western blot analysis:
Lane 1: EasyWestern Protein Standard (GenScript, MM0908)
Lane 2: Hela cell lysate

Primary antibody: 1 µg/ml Rabbit Anti-PGK1 Polyclonal Antibody (GenScript, A00961)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:5,000)

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Cat. No. A00961
40 ug $99.00