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Catalog Products » Other Products » New Products » Runx3 Antibody, pAb, Rabbit

Runx3 Antibody, pAb, Rabbit

Runx3 Antibody, PAb, Rabbit

Western blot analysis:
Lane 1: EasyWestern Protein Standard (GenScript, MM0908)
Lane 2: NIH3T3 cell lysate
Primary antibody: 1 µg/ml Rabbit Anti-Runx3 Polyclonal Antibody (GenScript, A00973)
Secondary antibody: Goat Anti-Rabbit IgG (H&L)[HRP] Polyclonal Antibody (GenScript, A00098, 1:5,000)

Runx3 Antibody, PAb, Rabbit

Runx3 antibody (A00973) western blot analysis in NIH/3T3 cell line lysates. This demonstrates the Runx3 antibody detected the Runx3 protein (arrow).

Synonyms Rabbit Anti Runx3 pAb;
Description Runx3 is also known as Core-binding factor, CBF-alpha 3, acute myeloid leukemia 2 protein, Oncogene AML-2, polyomavirus enhancer-binding protein 2 alpha C subunit, PEBP2-alpha C, PEA2-alpha. It binds to the core site of murine leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. Runx3 may be involved in the control of cellular proliferation and/or differentiation.
GenScript Rabbit Anti-Runx3 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide of mouse Runx3.
Host Species Rabbit
Antigen Species Mouse
Conjugation Unconjugated
Immunogen Synthetic peptide (KLH conjugated)
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide
Specificity GenScript Rabbit Anti-Runx3 Polyclonal Antibody can detect endogenous level of mouse Runx3. Not yet tested for other species.
Predicted Band Size 44 kD
Observed Band Size 44 kD
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.03-1 µg/ml
Western blot: 1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Runx3 Antibody, PAb, Rabbit
Runx3 Antibody, PAb, Rabbit

Western blot analysis:
Lane 1: EasyWestern Protein Standard (GenScript, MM0908)
Lane 2: NIH3T3 cell lysate
Primary antibody: 1 µg/ml Rabbit Anti-Runx3 Polyclonal Antibody (GenScript, A00973)
Secondary antibody: Goat Anti-Rabbit IgG (H&L)[HRP] Polyclonal Antibody (GenScript, A00098, 1:5,000)

Runx3 Antibody, PAb, Rabbit
Runx3 Antibody, PAb, Rabbit

Runx3 antibody (A00973) western blot analysis in NIH/3T3 cell line lysates. This demonstrates the Runx3 antibody detected the Runx3 protein (arrow).




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Cat. No. A00973
Size
Price
40 ug $99.00
 
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