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Catalog Products » Antibodies » Primary Antibodies » Neuroscience Antibodies » Superoxide Dismutase 1 Antibody, pAb, Rabbit

Superoxide Dismutase 1 Antibody, pAb, Rabbit

Synonyms Rabbit Anti-Superoxide Dismutase 1 pAb;Rabbit Anti Superoxide dismutase [Cu-Zn] pAb;Rabbit Anti SOD1 pAb;
Description Superoxide Dismutase 1 (SwissProt P00441) is a major antioxidant enzyme that catalyzes the conversion of superoxide anion to hydrogen peroxide and molecular oxygen. SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus, and mitochondrial intermembrane space. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1).
GenScript Rabbit Anti-Superoxide Dismutase 1 Polyclonal Antibody is developed in rabbit using a synthetic peptide of human Superoxide Dismutase 1.
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide (KLH conjugated) of human superoxide dismutase 1
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide
Specificity GenScript Rabbit Anti-Superoxide Dismutase 1 Polyclonal Antibody can detect endogenous level of human superoxide dismutase 1. Sequence homology predicts that it will also react with mouse, rat, pig, horse, bovine, bornean orangutan, chimpanzee, and fission yeast superoxide dismutase 1.
Predicted Band Size 18 kD
Observed Band Size 18 kD
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.03-1 μg/ml
Western blot: 1.0 µg/ml
Other applications: user-optimized

  • Superoxide Dismutase 1 Antibody, PAb, Rabbit
  • Superoxide Dismutase 1 Antibody, PAb, Rabbit

    Western blot analysis of cell lysates using Superoxide Dismutase 1 Antibody, pAb, Rabbit (GenScript, A01005, 1 µg/ml)
    The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
    Predicted Size: 18 kD
    Observed Size: 18 kD


Rajkumar S., et al. Exploration of Molecular Factors Impairing Superoxide Dismutase Isoforms Activity in Human Senile Cataractous Lenses. Invest Ophthalmol Vis Sci. 2013 Sep;54(9):6224-33.

NJ Dawson, et al. Free-radical first responders: The characterization of CuZnSOD and MnSOD regulation during freezing of the freeze-tolerant North American wood frog, Rana sylvatica. Biochim Biophys Acta. 2014Qct.;

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Cat. No. A01005
Size
Price
40 ug $99.00