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Resources » Research Applications » Stem Cell Research » Stem Cell Antibodies » WISP2 Antibody, mAb, Mouse

WISP2 Antibody, mAb, Mouse

Synonyms Mouse Anti-WISP2 mAb; Wnt-1-inducible signaling parthway protein-2
Description WISP-2 is a member of the connective tissue growth factor/cysteine-rich 61/nephroblastoma overexpressed (nov) (CCN) family and is upregulated in the mouse mammary epithelial cell line C57MG transformed by Wnt-1 and in several non-invasive human breast tumor cell lines. WISP-2 expression is enhanced by important modulators of human breast cancer cell proliferation such as estrogen, progesterone and epidermal growth factor (EGF) in MCF-7 cells. These effects, inhibited by appropriate antagonists, indicate that steroids and growth factor-induced upregulation of WISP-2 may be mediated through receptors. WISP2 gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.
Host Species Mouse
Antigen Species Human
Conjugation Unconjugated
Immunogen WISP2 full length recombinant protein with GST tag
Fusion Partner Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells.
Purification Protein A affinity column

Subclass Mouse IgG1
Clone ID 5D11E11
Concentration 1 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 1 mg/ml.
Species Reactivity GenScript Mouse Anti-WISP2 reacts with human WISP2. It has not yet been tested with other species.
Predicted Band Size 53 kD
Observed Band Size 53 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

  • WISP2 Antibody, MAb, Mouse
  • WISP2 Antibody, MAb, Mouse

    Lane 1,2,3 and 6: WISP2 full length recombinant protein with GST tag in 293 cell lysate
    Lane 4: 10 µg 293 cell lysate
    Lane 5: GST protein

    Primary antibody: A: 1 µg/ml Mouse Anti-WISP2 Monoclonal Antibody (GenScript, A01012)
    B: Negative control

    Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000)

    The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)




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Cat. No. A01012
Size
Price
40 ug $99.00