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Catalog Products » Antibodies » Primary Antibodies » Alzheimer's Disease Antibody list » Tau Antibody (Ser422), pAb, Rabbit

Tau Antibody (Ser422), pAb, Rabbit

Synonyms Rabbit Anti-Tau (Ab-422) Polyclonal Antibody;
Description Tau is a microtubule-associated phosphoprotein (MAP), localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms, which range in size from 352 to 441 amino acids. The tau variants differ from each other by the presence of either three or four repeat-regions in the carboxy-terminal part of the molecule and the absence or presence of one or two inserts in the amino-terminal portion.
Tau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5. At least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3β transfection phosphorylates serines 199, 202, 235, 396, 404, and 413, and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of tau. Phosphorylation on these sites reduces the ability of a given tau species to promote microtubule self-assembly. Hyperphosphorylated tau is the major protein of the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimer’s disease (AD). The PHFs are also found in the lesions of other central nervous system disorders.
GenScript Rabbit Anti-Tau (Ser422) Polyclonal Antibody is developed in rabbit using a synthetic peptide (KLH-coupled) corresponding to residues surrounding serine 422 of human tau.
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide (KLH-coupled) corresponding to residues surrounding serine 422 of human tau
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-Tau (Ser422) Polyclonal Antibody detects human tau protein, not yet tested for other species.
Species Reactivity Human
Predicted Band Size 85 kD
Observed Band Size 85 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2 µg/ml
Other applications: user optimized

  • Tau Antibody (Ser<sup>422</sup>), PAb, Rabbit
  • Tau Antibody (Ser<sup>422</sup>), PAb, Rabbit

    Western blot analysis of human recombinant tau fusion protein using Tau Antibody (Ser422), pAb, Rabbit (GenScript, A01051, 1μg/ml)
    The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
    Predicted Size: 85 kD
    Observed Size: 85 kD


Cat. No.
Product Name
Price
A00900-40
Tau Antibody (Phospho-Ser422), pAb, Rabbit - 40 ug
$99.00
 
TA Burrow, et al. CNS, Lung, and Lymph Node involvement in Gaucher disease type 3 after 11years therapy: Clinical, Histopathologic, and Biochemical Findings. Mol Genet Metab. 2014Sep.;

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Cat. No. A01051
Size
Price
40 ug $99.00