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Catalog Products » Antibodies » Primary Antibodies » Cytokine and Growth Factor Antibodies » GPR30 Antibody, pAb, Rabbit

GPR30 Antibody, pAb, Rabbit

Synonyms Rabbit Anti-GPR30 NT pAb;
Description GPR30 (G Protein-coupled Receptor 30), also known as G Protein-coupled estrogen Receptor 1 (GPER), is a member of G protein-coupled receptor family. It contains seven hydrophobic transmembrane domains embedded in hydrophilic intra- and extracellular loops. GPR30 transduces a variety of hormone, endogenous peptide, and neurotransmitter signals into intracellular effects via G proteins. GPR30 is an orphan receptor and localizes to the endoplasmic reticulum, where it specifically binds estrogen and estrogen derivatives. Activating GPR30 via estrogen results in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus.
GenScript Rabbit Anti-GPR30 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from amino terminal of human GPR30 protein (Swiss Prot: Q99527).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide from amino terminal of human GPR30 protein (Swiss Prot: Q99527)
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-GPR30 Polyclonal Antibody detects endogenous levels of human GPR30 protein.
Species Reactivity Human
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
IHC: 5-10 µg/ml
Other applications: user optimized.

  • GPR30 Antibody, PAb, Rabbit
  • GPR30 Antibody, PAb, Rabbit

    Immunohistochemistry analysis of human brain tissue slide (Paraffin embedded)
    using Rabbit Anti-GPR30 Polyclonal Antibody (Left, GenScript, A01074) and
    Purified Rabbit IgG (whole molecule) Control (Right, GenScript, A01008)




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Cat. No. A01074
Size
Price
40 ug $99.00