|Synonyms||Rabbit Anti-PIN1 pAb;|
|Description||PIN1 is an essential peptidyl-prolyl isomerase that regulates mitosis, presumably by interacting with NIMA and attenuating its mitosis-promoting activity. It displays a preference for an acidic residue N-terminal to the isomerized proline bond. It catalyzes pSer/Thr-Pro cis/trans isomerizations. PIN1 has been implicated in tau pathologies that underlie Alzheimer's disease. PIN1 binds to tau-phosphorylated sites, specifically on the Thr231-Pro site, where it induces conformational changes. PIN1 may be pivotal in maintaining normal neuronal function and preventing age-dependent neurodegeneration.
GenScript Rabbit Anti-PIN1 Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 50-100 of human PIN1 protein (Swiss Prot: Q13526).
|Immunogen||Synthetic peptide (KLH-coupled) within residues 50-100 of human PIN1 protein (Swiss Prot: Q13526)|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide|
|Reconstitution||Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.|
|Specificity||GenScript Rabbit Anti-PIN1 Polyclonal Antibody detects human PIN1 protein. Sequence homology predicts that it will also react with rat and mouse PIN1 proteins.|
|Species Reactivity||Human. This product has not yet been tested with other species.|
|Predicted Band Size||57 kD|
|Observed Band Size||57 kD|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Note||Small volumes of antibody will occasionally become trapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized
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