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Catalog Products » Antibodies » Primary Antibodies » Alzheimer's Disease Antibody list » Tau Antibody (Ser262), pAb, Rabbit

Tau Antibody (Ser262), pAb, Rabbit

Tau Antibody (Ser<sup>262</sup>), PAb, Rabbit

Western blot analysis of recombinant Human Tau protein using 1 µg/ml Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099)
Lane 1: Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099)
Lane 2: Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099) pre-incubated with non-phospho-peptide
Lane 3: Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099) pre-incubated with phospho-peptide
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Tau Antibody (Ser<sup>262</sup>), PAb, Rabbit

Western blot analysis of mouse brain tissue lysate using 1 µg/ml Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099)
Lane 1: Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099)
Lane 2: Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099) pre-incubated with non-phospho-peptide
Lane 3: Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099) pre-incubated with phospho-peptide
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

*This product is under development*
Synonyms Rabbit Anti-Tau (Ser262) pAb;
Description Tau is a microtubule-associated phosphoprotein (MAP) which is localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms ranging from 352 to 441 amino acids. The tau variant differs from each other with the presence or absence of either three or four repeated regions near the carboxy terminal of the molecule and one or two inserts near the amino terminal.
Tau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5. At least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3β transfection phosphorylates serines 199, 202, 235, 396, 404 and 413, and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of Tau. Phosphorylation on these sites reduces the ability of a given Tau species to promote microtubule self-assembly. Hyperphosphorylated Tau is the major protein forming the paired helical filaments (PHFs), which further form the pathological neurofibrillary tangles leading to Alzheimer’s disease (AD). These PHFs are also found to be related to the lesions of other central nervous system disorders.
GenScript Rabbit Anti-Tau (Ser262) Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide corresponding to the residues surrounding the serine 262 of human Tau.
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide (KLH-coupled) corresponding to residues surrounding the serine 262 of human Tau
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-Tau (Ser262) Polyclonal Antibody detects endogenous levels of total Tau protein.
Species Reactivity Human, mouse
Predicted Band Size 85 kD, 55 kD
Observed Band Size 85 kD, 55 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2 µg/ml
Other applications: user-optimized

  • Tau Antibody (Ser<sup>262</sup>), PAb, Rabbit
  • Tau Antibody (Ser<sup>262</sup>), PAb, Rabbit

    Western blot analysis of recombinant Human Tau protein using 1 µg/ml Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099)
    Lane 1: Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099)
    Lane 2: Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099) pre-incubated with non-phospho-peptide
    Lane 3: Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099) pre-incubated with phospho-peptide
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

  • Tau Antibody (Ser<sup>262</sup>), PAb, Rabbit
  • Tau Antibody (Ser<sup>262</sup>), PAb, Rabbit

    Western blot analysis of mouse brain tissue lysate using 1 µg/ml Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099)
    Lane 1: Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099)
    Lane 2: Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099) pre-incubated with non-phospho-peptide
    Lane 3: Rabbit Anti-Tau (Ser262) Polyclonal Antibody (GenScript, A01099) pre-incubated with phospho-peptide
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.




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