Tau Antibody (Phospho-Thr205), pAb, Rabbit Antibody

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Overview

Synonyms	Rabbit Anti-Tau (Phospho-Thr²⁰⁵) pAb;
Description	Tau is a microtubule-associated phosphoprotein (MAP), localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms, which range from 352 to 441 amino acids in size. These tau variants differ from each other in the presence of either three or four repeat-regions in the carboxy-terminal part of the molecule and the presence or absence of one or two inserts in the amino-terminal portion. Tau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5. At least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3β transfection phosphorylates serines 199, 202, 235, 396, 404 and 413, and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of tau. Phosphorylation on these sites reduces the ability of a given tau species to promote microtubule self-assembly. Hyperphosphorylated tau is the major protein of the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimer’s disease (AD). These PHFs are also found in the lesions of other central nervous system disorders. GenScript Rabbit Anti-Tau (Phospho-Thr²⁰⁵) Polyclonal Antibody is developed in rabbit hosts using a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding threonine 205 of human tau.
Host Species	Rabbit
Antigen Species	Human
Conjugation	Unconjugated
Immunogen	Synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding phospho-threonine 205 of human tau
Purification	Affinity chromatography using phosphopeptide, cross-adsorbed with the corresponding non-phosphopeptide

Properties

Subclass	Rabbit IgG
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity	GenScript Rabbit Anti-Tau (Phospho-Thr²⁰⁵) Polyclonal Antibody detects endogenous levels of tau protein only when phosphorylated at threonine 205.
Species Reactivity	Human, mouse
Predicted Band Size	85 kD
Observed Band Size	55 kD
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2 µg/ml
Other applications: user-optimized

Examples

Tau Antibody (Phospho-Thr<sup>205</sup>), PAb, Rabbit

Western blot analysis of mouse brain tissue lysate, using 2 µg/ml Rabbit Anti-Tau (Phospho-Thr²⁰⁵) Polyclonal Antibody (GenScript, A01100)
Lane 1: Rabbit Anti-Tau (Phospho-Thr²⁰⁵) Polyclonal Antibody
Lane 2: Rabbit Anti-Tau (Phospho-Thr²⁰⁵) Polyclonal Antibody pre-incubated with non-phospho-peptide
Lane 3: Rabbit Anti-Tau (Phospho-Thr²⁰⁵) Polyclonal Antibody pre-incubated with phospho-peptide
Lane 4: Rabbit Anti-Tau (Phospho-Thr²⁰⁵) Polyclonal Antibody pre-incubated with generic phospho-threonine containing peptide
The signal was developed with IRDye^TM 800 Conjugated Goat Anti-Rabbit IgG