|Synonyms||Rabbit Anti-Histone 2B pAb;|
|Description||Histone 2B is the core component of nucleosome. It is involved in nucleosome wrapping and the compaction of DNA into chromatin, limiting DNA accessibility. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex system of nucleosome remodelling and post-translational modification of histones, also called histone code.
GenScript Rabbit Anti-Histone 2B Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide corresponding to carboxyl terminal residues of human histone 2B protein (Swiss Prot: Q99879).
|Immunogen||Synthetic peptide (KLH-coupled) derived from the carboxyl terminal of human histone 2B (Swiss Prot: Q99879).|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide|
|Reconstitution||Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.|
|Specificity||GenScript Rabbit Anti-Histone 2B Polyclonal Antibody detects endogenous levels of human histone 2B protein. Sequence homology predicts that it will also react with Histone 2B protein within mouse and fruit fly proteins.|
|Predicted Band Size||14 kD|
|Observed Band Size||18 kD|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|The investigator must determine the working concentrations for each specific application. The appropriate concentration may depend on such factors as secondary antibody affinity, antigen concentration, detection method, temperature, and length of incubation. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized
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