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Home » Gene Synthesis & Molecular Biology Services » Histone 2B Antibody, pAb, Rabbit

Histone 2B Antibody, pAb, Rabbit

Synonyms Rabbit Anti-Histone 2B pAb;
Description Histone 2B is the core component of nucleosome. It is involved in nucleosome wrapping and the compaction of DNA into chromatin, limiting DNA accessibility. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex system of nucleosome remodelling and post-translational modification of histones, also called histone code.
GenScript Rabbit Anti-Histone 2B Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide corresponding to carboxyl terminal residues of human histone 2B protein (Swiss Prot: Q99879).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide (KLH-coupled) derived from the carboxyl terminal of human histone 2B (Swiss Prot: Q99879).
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-Histone 2B Polyclonal Antibody detects endogenous levels of human histone 2B protein. Sequence homology predicts that it will also react with Histone 2B protein within mouse and fruit fly proteins.
Species Reactivity Human
Predicted Band Size 14 kD
Observed Band Size 18 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

The investigator must determine the working concentrations for each specific application. The appropriate concentration may depend on such factors as secondary antibody affinity, antigen concentration, detection method, temperature, and length of incubation. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized

  • Histone 2B Antibody, PAb, Rabbit
  • Histone 2B Antibody, PAb, Rabbit

    Western blot analysis of Hela cell lysate using 1 µg/ml Rabbit Anti-Histone 2B Polyclonal Antibody (GenScript, A01174)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Cat. No.
Product Name
Price
A01176-40
Histone H4 Antibody, pAb, Rabbit - 40 ug
$99.00
 


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Cat. No. A01174
Size
Price
40 ug $99.00