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Catalog Products » Other Products » Catalog Peptides » All Catalog Peptides » Catalase Antibody, pAb, Rabbit

Catalase Antibody, pAb, Rabbit

Catalase Antibody, PAb, Rabbit

Western blot analysis of mouse liver tissue lysate using 1 µg/ml Rabbit Anti-Catalase Polyclonal Antibody (GenScript, A01187)
Lane 1. Rabbit Anti-Catalase Polyclonal Antibody
Lane 2. Rabbit Anti-Catalase Polyclonal Antibody pre-incubated with immunizing peptide
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Catalase Antibody, PAb, Rabbit

Western blot analysis of Hela cell lysate using 2 µg/ml Rabbit Anti-Catalase Polyclonal Antibody (GenScript, A01187)
Lane 1. Rabbit Anti-Catalase Polyclonal Antibody
Lane 2. Rabbit Anti-Catalase Polyclonal Antibody pre-incubated with immunizing peptide
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.

Catalase Antibody, PAb, Rabbit

Western blot analysis of immunoprecipitates from mouse liver lysate using Rabbit Anti-Catalase Polyclonal Antibody (GenScript, A01187).
Lane 1: Input control material for mouse liver lysate
Lane 2: Negative control – IP with isotype control antibody (GenScript, A01008)
Lane 3: Immunprecipitation with Rabbit Anti-Catalase Polyclonal Antibody (GenScript, A01187).

Catalase Antibody, PAb, Rabbit

Flow cytometric analysis of Ramos cells using Catalase Antibody, pAb, Rabbit (GenScript, A01187; shaded histogram) or with an isotype
control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

Synonyms Rabbit anti Catalase(amino-terminal) polyclonal antibody;
Description Catalase, also known as CAT, is the classical marker for peroxisomes, in which it is the most abundant protein. It is found in almost all aerobically respiring organisms and serves to protect cells from hydrogen peroxide toxicity. Defects in CAT cause acatalasia, which is characterized by the absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
GenScript Catalase Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide derived from the residues near the carboxyl terminus of the human catalase protein (Swiss Prot: P04040).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide (KLH-coupled) derived from residues near the carboxyl terminus of the human catalase protein (Swiss Prot: P04040)
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Catalase Antibody, pAb, Rabbit detects endogenous levels of human and mouse catalase protein.
Species Reactivity Human, mouse. Reactivity within other species has not been tested yet.
Predicted Band Size 60 kD
Observed Band Size 60 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2 µg/ml
Immunoprecipitation (IP): 2-10 µg/mg of lysate
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user optimized

  • Catalase Antibody, PAb, Rabbit
  • Catalase Antibody, PAb, Rabbit

    Western blot analysis of mouse liver tissue lysate using 1 µg/ml Rabbit Anti-Catalase Polyclonal Antibody (GenScript, A01187)
    Lane 1. Rabbit Anti-Catalase Polyclonal Antibody
    Lane 2. Rabbit Anti-Catalase Polyclonal Antibody pre-incubated with immunizing peptide
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

  • Catalase Antibody, PAb, Rabbit
  • Catalase Antibody, PAb, Rabbit

    Western blot analysis of Hela cell lysate using 2 µg/ml Rabbit Anti-Catalase Polyclonal Antibody (GenScript, A01187)
    Lane 1. Rabbit Anti-Catalase Polyclonal Antibody
    Lane 2. Rabbit Anti-Catalase Polyclonal Antibody pre-incubated with immunizing peptide
    The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.

  • Catalase Antibody, PAb, Rabbit
  • Catalase Antibody, PAb, Rabbit

    Western blot analysis of immunoprecipitates from mouse liver lysate using Rabbit Anti-Catalase Polyclonal Antibody (GenScript, A01187).
    Lane 1: Input control material for mouse liver lysate
    Lane 2: Negative control – IP with isotype control antibody (GenScript, A01008)
    Lane 3: Immunprecipitation with Rabbit Anti-Catalase Polyclonal Antibody (GenScript, A01187).

  • Catalase Antibody, PAb, Rabbit
  • Catalase Antibody, PAb, Rabbit

    Flow cytometric analysis of Ramos cells using Catalase Antibody, pAb, Rabbit (GenScript, A01187; shaded histogram) or with an isotype
    control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.


Rouleau M., et al. Dual Roles for Splice Variants of the Glucuronidation Pathway as Regulators of Cellular Metabolism. Mol Pharmacol. 2014 Jan;85(1):29-36.

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Cat. No. A01187
Size
Price
40 ug $99.00