|Synonyms||Rabbit anti Calnexin Polyclonal Antibody;|
|Description||Calnexin is a calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. As a chaperone protein, it assists in protein assembly by ensuring that only the properly folded protein is progresses along the secretory pathway. It seems to play a major role in the quality control apparatus of the ER by retaining incorrectly folded proteins.
GenScript Rabbit Anti-Calnexin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 250-300 of human calnexin protein (Swiss Prot: P27824).
|Immunogen||Synthetic peptide (KLH-coupled) within residues 250-300 of human calnexin (Swiss Prot: P27824)|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide|
|Reconstitution||Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.|
|Specificity||GenScript Rabbit Anti-Calnexin Polyclonal Antibody detects endogenous levels of human calnexin protein. Sequence homology predicts that it will also react with mouse and rat calnexin.|
|Species Reactivity||Human. This product has not yet been tested with other species.|
|Predicted Band Size||90 kD|
|Observed Band Size||85 kD|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized
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