• English
  • Sign In
  • Contact Us
×
Resources » Research Applications » Stem Cell Research » Stem Cell Antibodies » Calnexin Antibody, pAb, Rabbit

Calnexin Antibody, pAb, Rabbit

Synonyms Rabbit anti Calnexin Polyclonal Antibody;
Description Calnexin is a calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. As a chaperone protein, it assists in protein assembly by ensuring that only the properly folded protein is progresses along the secretory pathway. It seems to play a major role in the quality control apparatus of the ER by retaining incorrectly folded proteins.

GenScript Rabbit Anti-Calnexin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 250-300 of human calnexin protein (Swiss Prot: P27824).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide (KLH-coupled) within residues 250-300 of human calnexin (Swiss Prot: P27824)
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-Calnexin Polyclonal Antibody detects endogenous levels of human calnexin protein. Sequence homology predicts that it will also react with mouse and rat calnexin.
Species Reactivity Human. This product has not yet been tested with other species.
Predicted Band Size 90 kD
Observed Band Size 85 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized

  • Calnexin Antibody, PAb, Rabbit
  • Calnexin Antibody, PAb, Rabbit

    Western blot analysis of cell lysates, using Calnexin Antibody, pAb, Rabbit (GenScript, A01192, 1 µg/ml)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 90 kD
    Observed Size: 85 kD




Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. A01192
Size
Price
40 ug $99.00