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Resources » Research Applications » Stem Cell Research » Stem Cell Antibodies » Vimentin Antibody, pAb, Rabbit

Vimentin Antibody, pAb, Rabbit

Vimentin Antibody, PAb, Rabbit

Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-Vimentin Polyclonal Antibody (GenScript, A01193)
Lane 1, 3: Hela cell lysate
Lane 2, 4: HEK293 cell lysate
Primary antibody:
Lane 1, 2: Rabbit Anti-Vimentin Polyclonal Antibody
Lane 3, 4: Rabbit Anti-Vimentin Polyclonal Antibody pre-incubated with immunizing peptide
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted band size 53 kD
Observed band size 53 kD
Additional band size 30 kD (Possible vimentin fragment)

Vimentin Antibody, PAb, Rabbit

Immunohistochemistry analysis of human spleen tissue slide (Paraffin embedded) using Rabbit Anti-Vimentin Polyclonal Antibody (Left, GenScript, A01193)
and Purified Rabbit IgG (Whole molecule) Control (Right, GenScript, A01008)

Vimentin Antibody, PAb, Rabbit

Flow cytometric analysis of Ramos cells using Vimentin Antibody, pAb, Rabbit (GenScript, A01193; shaded histogram) or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

Synonyms Rabbit anti Vimentin (400-500 aa);
Description Vimentin is a member of the intermediate filament protein family. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Despite the fact that most intermediate filaments exist as stable structures, in fibroblasts, vimentin take on a dynamic structure.
GenScript Vimentin Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 400-500 of human vimentin protein (Swiss Prot: P08670).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide (KLH-coupled) derived from within residues 400-500 of human vimentin (Swiss Prot: P08670)
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity GenScript Vimentin Antibody, pAb, Rabbit detects endogenous levels of human vimentin protein. Sequence homology predicts that it will also react with mouse and rat vimentin.
Species Reactivity Human. This product has not yet been tested with other species.
Predicted Band Size 53 kD
Observed Band Size 53 kD, 30 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Immunohistochemistry: 5-10 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user-optimized

  • Vimentin Antibody, PAb, Rabbit
  • Vimentin Antibody, PAb, Rabbit

    Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-Vimentin Polyclonal Antibody (GenScript, A01193)
    Lane 1, 3: Hela cell lysate
    Lane 2, 4: HEK293 cell lysate
    Primary antibody:
    Lane 1, 2: Rabbit Anti-Vimentin Polyclonal Antibody
    Lane 3, 4: Rabbit Anti-Vimentin Polyclonal Antibody pre-incubated with immunizing peptide
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted band size 53 kD
    Observed band size 53 kD
    Additional band size 30 kD (Possible vimentin fragment)

  • Vimentin Antibody, PAb, Rabbit
  • Vimentin Antibody, PAb, Rabbit

    Immunohistochemistry analysis of human spleen tissue slide (Paraffin embedded) using Rabbit Anti-Vimentin Polyclonal Antibody (Left, GenScript, A01193)
    and Purified Rabbit IgG (Whole molecule) Control (Right, GenScript, A01008)

  • Vimentin Antibody, PAb, Rabbit
  • Vimentin Antibody, PAb, Rabbit

    Flow cytometric analysis of Ramos cells using Vimentin Antibody, pAb, Rabbit (GenScript, A01193; shaded histogram) or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.




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Cat. No. A01193
Size
Price
40 ug $49.5
$99.00