|Synonyms||Rabbit Anti-AHA1 pAb;|
|Description||AHA1 is a cochaperone that stimulates HSP90 ATPase activity. It may affect a step in the endoplasmic reticulum to Golgi trafficking. AHA1 interacts with HSPCA/HSP90 and the cytoplasmic tail of the vesicular stomatitis virus glycoprotein. The protein is expressed in numerous tissues, including brain, heart, skeletal muscle, kidney, liver, and placental tissues.
GenScript Rabbit Anti-AHA1 Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within the carboxyl terminus of human AHA1 protein (Swiss Prot: O95433).
|Immunogen||Synthetic peptide (KLH-coupled) within carboxyl terminal of human AHA1 protein (Swiss Prot: O95433)|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide|
|Reconstitution||Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.|
|Specificity||GenScript Rabbit Anti-AHA1 Polyclonal Antibody detects human AHA1 protein. Sequence homology predicts that it will also react with rat and mouse AHA1 proteins.|
|Species Reactivity||Human. This product has not yet been tested with other species.|
|Predicted Band Size||38 kD|
|Observed Band Size||38 kD|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)
|Cat. No. A01197|