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AHA1 Antibody, pAb, Rabbit

Synonyms Rabbit Anti-AHA1 pAb;
Description AHA1 is a cochaperone that stimulates HSP90 ATPase activity. It may affect a step in the endoplasmic reticulum to Golgi trafficking. AHA1 interacts with HSPCA/HSP90 and the cytoplasmic tail of the vesicular stomatitis virus glycoprotein. The protein is expressed in numerous tissues, including brain, heart, skeletal muscle, kidney, liver, and placental tissues.
GenScript Rabbit Anti-AHA1 Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within the carboxyl terminus of human AHA1 protein (Swiss Prot: O95433).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide (KLH-coupled) within carboxyl terminal of human AHA1 protein (Swiss Prot: O95433)
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-AHA1 Polyclonal Antibody detects human AHA1 protein. Sequence homology predicts that it will also react with rat and mouse AHA1 proteins.
Species Reactivity Human. This product has not yet been tested with other species.
Predicted Band Size 38 kD
Observed Band Size 38 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

AHA1 Antibody, PAb, Rabbit
AHA1 Antibody, PAb, Rabbit

Western blot analysis of recombinant human AHA1 protein using 1 µg/ml Rabbit Anti-AHA1 Polyclonal Antibody (GenScript, A01197)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG

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Cat. No. A01197
40 ug $99.00
24 hour