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Home » Gene Synthesis & Molecular Biology Services » Calcineurin A Antibody, pAb, Rabbit

Calcineurin A Antibody, pAb, Rabbit

Calcineurin A Antibody, PAb, Rabbit

Western blot analysis of tissue lysates using Calcineurin A Antibody, pAb, Rabbit (GenScript, A01200, 0.5 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG
Predicted Size: 59 kD
Observed Size: 60 kD, 32kD

Calcineurin A Antibody, PAb, Rabbit

Western blot analysis of human recombinant Calcineurin A fusion protein using Calcineurin A Antibody, pAb, Rabbit (GenScript, A01200, 0.5 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG
Predicted Size: 115 kD
Observed Size: 115 kD

Synonyms Rabbit Anti-Calcineurin A pAb;
Description Calcineurin is a heterodimer protein that binds to Ca2+. A Ca2+/calmodulin-regulated protein phosphatase, it is composed of a 19 kD calcineurin B (CnB) and a 61 kD calmodulin-binding catalytic subunit, calcineurin A (CnA). Calcineurin A was first detected in skeletal muscle and brain tissues, but it has since been found in all cell types, from yeast to mammalian. Calcineurin A may be responsible for the calmodulin activation property of calcineurin. There is evidence suggesting that alternative splicing could give rise to different forms of calcineurin A.
GenScript Rabbit Anti-Calcineurin A Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within N-terminal of human calcineurin A protein (Swiss Prot: Q08209).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide (KLH-coupled) within the N-terminal of human calcineurin A protein (Swiss Prot: Q08209).
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-Calcineurin A Polyclonal Antibody detects endogenous levels of calcineurin A protein. Sequence homology predicts that it will also react with rat calcineurin A.
Species Reactivity Human and mouse. This product has not yet been tested with other species.
Predicted Band Size 59 kD
Observed Band Size 115 kD, 60 kD, 32 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volumes of antibody will occasionally become trapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Immunoprecipitation: 2-10 µg/mg of lysate
Other applications: user-optimized

  • Calcineurin A Antibody, PAb, Rabbit
  • Calcineurin A Antibody, PAb, Rabbit

    Western blot analysis of tissue lysates using Calcineurin A Antibody, pAb, Rabbit (GenScript, A01200, 0.5 µg/ml)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG
    Predicted Size: 59 kD
    Observed Size: 60 kD, 32kD

  • Calcineurin A Antibody, PAb, Rabbit
  • Calcineurin A Antibody, PAb, Rabbit

    Western blot analysis of human recombinant Calcineurin A fusion protein using Calcineurin A Antibody, pAb, Rabbit (GenScript, A01200, 0.5 µg/ml)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG
    Predicted Size: 115 kD
    Observed Size: 115 kD


Cat. No.
Product Name
Price
A01199-40
Calcineurin A Antibody, pAb, Rabbit - 40 ug
$99.00
 


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Cat. No. A01200
Size
Price
40 ug $99.00