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Catalog Products » Antibodies » Primary Antibodies » Control Antibodies » β-Tubulin III Antibody, pAb, Rabbit

β-Tubulin III Antibody, pAb, Rabbit

β-Tubulin III Antibody, PAb, Rabbit

Lane 1: Marker
Lane 2: Hela cell lysate
Lane 3: HEK293 cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate
Western blot analysis of lysates using 1 µg/ml Rabbit Anti-β-Tubulin III Polyclonal Antibody (GenScript, A01203)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

β-Tubulin III Antibody, PAb, Rabbit

Immunohistochemistry analysis of human brain tissue slide (Paraffin embedded) using
Rabbit Anti-β-Tubulin III Polyclonal Antibody (GenScript, A01203)

β-Tubulin III Antibody, PAb, Rabbit

Immunohistochemistry negative control analysis of human brain tissue slide (Paraffin embedded) using
Purified Rabbit IgG (Whole Molecule) Control (GenScript, A01008)

β-Tubulin III Antibody, PAb, Rabbit

Lane 1: Marker
Lane 2: Hela cell lysate
Lane 3: HEK293 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: K562 cell lysate
Lane 6: Ramos cell lysate
Lane 7: A549 cell lysate
Lane 8: HepG2 cell lysate
Lane 9: Mouse brain tissue lysate
Lane 10: Rat brain tissue lysate
Western blot analysis of lysates using 1 µg/ml Rabbit Anti-β-Tubulin III Polyclonal Antibody (GenScript, A01203)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

β-Tubulin III Antibody, PAb, Rabbit

Flow cytometric analysis of HeLa cells using Rabbit Anti-β-Tubulin III Polyclonal Antibody (GenScript, A01203; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

β-Tubulin III Antibody, PAb, Rabbit

Flow cytometric analysis of Jurkat cells using Rabbit Anti-β-Tubulin III Polyclonal Antibody (GenScript, A01203; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by FITC conjugated anti-rabbit IgG.

Synonyms Rabbit Anti-Beta-Tubulin III pAb;
Description β-Tubulin III belongs to the tubulin family. Tubulin is the major constituent of microtubules. It forms dimers of alpha and beta chains. One mole of tubulin binds to two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. β-Tubulin III contains a highly acidic C-terminal region which can bind cations such as calcium. It is abundant in the central and peripheral nervous systems.
GenScript Rabbit Anti-β-Tubulin III Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 150-200 of human β-Tubulin III protein (Swiss Prot: Q13509).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide (KLH-coupled) within residues 150-200 of human β-tubulin III protein (Swiss Prot: Q13509)
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-β-Tubulin III Polyclonal Antibody detects endogenous levels of human, rat and mouse β-tubulin III protein. Sequence homology predicts that it will also react with bovine, chicken, cynomolgus monkey. African clawed frog, and disc abalone β-tubulin III proteins.
Species Reactivity Human, mouse, and rat. This product has not yet been tested with other species. Reactivity with other β-Tubulin had not been tested yet.
Predicted Band Size 50 kD
Observed Band Size 50 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Immunohistochemistry: 5-10 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

β-Tubulin III Antibody, PAb, Rabbit
β-Tubulin III Antibody, PAb, Rabbit

Lane 1: Marker
Lane 2: Hela cell lysate
Lane 3: HEK293 cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate
Western blot analysis of lysates using 1 µg/ml Rabbit Anti-β-Tubulin III Polyclonal Antibody (GenScript, A01203)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

β-Tubulin III Antibody, PAb, Rabbit
β-Tubulin III Antibody, PAb, Rabbit

Immunohistochemistry analysis of human brain tissue slide (Paraffin embedded) using
Rabbit Anti-β-Tubulin III Polyclonal Antibody (GenScript, A01203)

β-Tubulin III Antibody, PAb, Rabbit
β-Tubulin III Antibody, PAb, Rabbit

Immunohistochemistry negative control analysis of human brain tissue slide (Paraffin embedded) using
Purified Rabbit IgG (Whole Molecule) Control (GenScript, A01008)

β-Tubulin III Antibody, PAb, Rabbit
β-Tubulin III Antibody, PAb, Rabbit

Lane 1: Marker
Lane 2: Hela cell lysate
Lane 3: HEK293 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: K562 cell lysate
Lane 6: Ramos cell lysate
Lane 7: A549 cell lysate
Lane 8: HepG2 cell lysate
Lane 9: Mouse brain tissue lysate
Lane 10: Rat brain tissue lysate
Western blot analysis of lysates using 1 µg/ml Rabbit Anti-β-Tubulin III Polyclonal Antibody (GenScript, A01203)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

β-Tubulin III Antibody, PAb, Rabbit
β-Tubulin III Antibody, PAb, Rabbit

Flow cytometric analysis of HeLa cells using Rabbit Anti-β-Tubulin III Polyclonal Antibody (GenScript, A01203; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

β-Tubulin III Antibody, PAb, Rabbit
β-Tubulin III Antibody, PAb, Rabbit

Flow cytometric analysis of Jurkat cells using Rabbit Anti-β-Tubulin III Polyclonal Antibody (GenScript, A01203; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by FITC conjugated anti-rabbit IgG.


Cat. No.
Product Name
Size
Price
Quantity
A01203-40 β-Tubulin III Antibody, pAb, Rabbit
40 ug
$99.00
A01410-100 THETM alpha Tubulin Antibody, mAb, Mouse
100 ug
$225.00
A01410-40 THETM alpha Tubulin Antibody, mAb, Mouse
40 ug
$108.00
A01490 THETM alpha Tubulin Antibody [HRP], mAb, Mouse
100 ug
$335.00
A01545 THETM alpha Tubulin Antibody [Biotin], mAb, Mouse
100 ug
$335.00
A01627-40 Neuron specific β-Tubulin III Antibody, pAb, Rabbit
40 ug
$99.00
A01717-40 β-tubulin Antibody (2G7D4), mAb, Mouse
40 ug
$99.00
 
Yang J., et al. Inhibition of farnesyl pyrophosphate synthase attenuates angiotensin II-induced cardiac hypertrophy and fibrosis in vivo. Int J Biochem Cell Biol. 2013 Mar;45(3):657-66.

K Hasegawa., et al. Chromosomal gain promotes formation of a steep RanGTP gradient that drives mitosis in aneuploid cells. J Cell Biol. 2013 Jan;200(2):151-61.

Das S., et al. Oncogenic Splicing Factor SRSF1 Is a Critical Transcriptional Target of MYC. Cell Rep. 2012 Feb;1(2):110-7.

Simo L., et al. Invertebrate specific D1‐like dopamine receptor in control of salivary glands in the black‐legged tick Ixodes scapularis. J Comp Neurol. 2013 Dec;10.1002/cne.23515.

Shi Z., et al. Effect of All-Trans Retinoic Acid on the Differentiation of U87 Glioma Stem/Progenitor Cells. Cell Mol Neurobiol. 2013 Oct;33(7):943-51.

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Cat. No. A01203
Size
Price
40 ug $99.00
24 hour
 
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