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Home » Gene Synthesis & Molecular Biology Services » 5HT5A Antibody, pAb, Rabbit

5HT5A Antibody, pAb, Rabbit

5HT5A Antibody, PAb, Rabbit

Western blot analysis of lysates using 1 µg/ml Rabbit Anti-5HT5A Polyclonal Antibody (GenScript, A01209)
Lane 1: Hela cell lysate
Lane 2: Mouse brain tissue lysate
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

5HT5A Antibody, PAb, Rabbit

Flow cytometric analysis of Ramos cells using 5HT5A Antibody, pAb, Rabbit (GenScript, A01209; shaded histogram) or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

Synonyms Rabbit Anti 5TH5A polyclonal antibody; Rabbit Anti 5TH5A receptor polyclonal antibody
Description 5HT5A receptor is a G protein coupled receptor (GPCR) in the serotonin receptor family. HT receptors interact with the neurotransmitter and peripheral signal mediator serotonin. In particular, 5-hydroxytryptamine (5HT) receptors mediate the effects of serotonin in its role as the endogenous ligand and in pharmaceutical and hallucinogenic drugs. In addition, 5HT receptors affect the release and activities of other neurotransmitters such as glutamate, dopamine, and GABA.
GenScript 5HT5A Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide derived from the 3rd intracellular domain loop of human 5HT5A Receptor (Swiss Prot: P47898).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide (KLH-coupled) derived from the 3rd intracellular domain loop of human 5HT5A receptor (Swiss Prot P47898)
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity GenScript 5HT5A Antibody, pAb, Rabbit detects endogenous levels of human and mouse 5HT5A protein.
Species Reactivity Human, mouse. This product has not yet been tested with other species.
Predicted Band Size 40 kD
Observed Band Size 40 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user-optimized

  • 5HT5A Antibody, PAb, Rabbit
  • 5HT5A Antibody, PAb, Rabbit

    Western blot analysis of lysates using 1 µg/ml Rabbit Anti-5HT5A Polyclonal Antibody (GenScript, A01209)
    Lane 1: Hela cell lysate
    Lane 2: Mouse brain tissue lysate
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

  • 5HT5A Antibody, PAb, Rabbit
  • 5HT5A Antibody, PAb, Rabbit

    Flow cytometric analysis of Ramos cells using 5HT5A Antibody, pAb, Rabbit (GenScript, A01209; shaded histogram) or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.




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Cat. No. A01209
Size
Price
40 ug $99.00