• English
  • Sign In
  • Contact Us
×
Resources » Research Applications » Stem Cell Research » Stem Cell Antibodies » Calnexin Antibody, pAb, Rabbit

Calnexin Antibody, pAb, Rabbit

Calnexin Antibody, PAb, Rabbit

Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HEK293 cell lysate
Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-Calnexin Polyclonal Antibody (GenScript, A01234)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Calnexin Antibody, PAb, Rabbit

Flow cytometric analysis of HepG2 cells using Calnexin Antibody, pAb, Rabbit (GenScript, A01234; shaded histogram) or with an isotype
control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

Synonyms Rabbit Anti Calnexin (N-terminal) Polyclonal Antibody;
Description Calnexin is a calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may be involved in assisting protein assembly or in the retention within the endoplasmic reticulum (ER) of unassembled protein subunits. It seems to play a major role in the ER quality control apparatus via the retention of incorrectly folded proteins.

GenScript Calnexin Antibody, pAb, Rabbit is developed in rabbit hosts using a KLH-coupled synthetic peptide from within N-terminal of human calnexin protein (Swiss Prot: P27824).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide from within N-terminal of human calnexin (Swiss Prot: P27824)
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Calnexin Antibody, pAb, Rabbit detects endogenous levels of human calnexin protein.
Species Reactivity Human
Predicted Band Size 90 kD
Observed Band Size 85 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volumes of antibody will occasionally become trapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user optimized

  • Calnexin Antibody, PAb, Rabbit
  • Calnexin Antibody, PAb, Rabbit

    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: HEK293 cell lysate
    Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-Calnexin Polyclonal Antibody (GenScript, A01234)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

  • Calnexin Antibody, PAb, Rabbit
  • Calnexin Antibody, PAb, Rabbit

    Flow cytometric analysis of HepG2 cells using Calnexin Antibody, pAb, Rabbit (GenScript, A01234; shaded histogram) or with an isotype
    control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.


Wu H., et al. Transcriptional Suppression of CYP2A13 Expression by Lipopolysaccharide in Cultured Human Lung Cells and the Lungs of a CYP2A13-Humanized Mouse Model. Toxicol Sci. 2013 Oct;135(2):476-85.

Nechaev S., et al. Intracellular processing of immunostimulatory CpG-siRNA: Toll-like receptor 9 facilitates siRNA dicing and endosomal escape. J Control Release. 2013 Sep;170(3):307-15.

Yuan Wei., et al. Generation And Characterization Of A Novel Cyp2A(4/5)Bgs-Null Mouse Model. Drug Metab Dispos. 2013 Jan;41(1):132 - 140.

Wei Y., et al. Generation And Characterization Of A Cyp2A13/2B6/2F1-Transgenic Mouse Model. Drug Metab Dispos. 2012 Jun;40(6):1144-50.

Fang Xie., et al. The Tissue-Specific Toxicity of Methimazole in the Mouse Olfactory Mucosa Is Partly Mediated through Target-Tissue Metabolic Activation by CYP2A5. Drug Metab Dispos. 2011 Jun;39(6):947 - 951.

You can visit "Citations Database" for more citations.






For more documents, please visit "Technical Support".

Cat. No. A01234
Size
Price
40 ug $99.00