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Resources » Research Applications » Stem Cell Research » Stem Cell Antibodies » Calnexin Antibody, pAb, Rabbit

Calnexin Antibody, pAb, Rabbit

Calnexin Antibody, PAb, Rabbit

Western blot analysis of cell lysates, using Calnexin Antibody, pAb, Rabbit (GenScript, A01240, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 90 kD
Observed Size: 85 kD

Calnexin Antibody, PAb, Rabbit

Flow cytometric analysis of Ramos cells using Calnexin Antibody, pAb, Rabbit (GenScript, A01240; shaded histogram) or with an isotype
control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

Synonyms Rabbit Anti-Calnexin pAb;
Description Calnexin is calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may be involved in assisting protein assembly or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the ER quality control apparatus via the retention of incorrectly folded proteins.
GenScript Calnexin Antibody, pAb, Rabbit is developed in rabbit hosts using a KLH-coupled synthetic peptide from carboxyl terminal of human calnexin protein (Swiss Prot: P27824).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide (KLH-coupled) from carboxyl terminal of human calnexin (Swiss Prot: P27824).
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Calnexin Antibody, pAb, Rabbit detects endogenous levels of human and mouse calnexin protein. Sequence homology predicts that it will also react with rat, bovine, canine, and Sumatran orangutan calnexin.
Species Reactivity Human and mouse. This product has not yet been tested with other species.
Predicted Band Size 90 kD
Observed Band Size 85 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user-optimized

  • Calnexin Antibody, PAb, Rabbit
  • Calnexin Antibody, PAb, Rabbit

    Western blot analysis of cell lysates, using Calnexin Antibody, pAb, Rabbit (GenScript, A01240, 1 µg/ml)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 90 kD
    Observed Size: 85 kD

  • Calnexin Antibody, PAb, Rabbit
  • Calnexin Antibody, PAb, Rabbit

    Flow cytometric analysis of Ramos cells using Calnexin Antibody, pAb, Rabbit (GenScript, A01240; shaded histogram) or with an isotype
    control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.


Li Q., et al. Estradiol potentiates 8-OH-DPAT-induced sumoylation of 5-HT1A receptor: Characterization and subcellular distribution of sumoylated 5-HT1A receptors. Psychoneuroendocrinology. 2013 Nov;38(11):2542-53.

Weber MM., et al. Identification of Coxiella burnetii Type IV Secretion Substrates Required for Intracellular Replication and Coxiella-Containing Vacuole Formation. J Bacteriol. 2013 Sep;195(17):3914-24.

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Cat. No. A01240
Size Price
40 ug $99.00