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Home » Gene Synthesis & Molecular Biology Services » HSP70 Antibody, pAb, Rabbit

HSP70 Antibody, pAb, Rabbit

HSP70 Antibody, PAb, Rabbit

Western blot analysis of Hela cell lysate using 1 µg/ml Rabbit Anti-HSP70 Polyclonal Antibody (GenScript, A01243)
Lane 1: Rabbit Anti-HSP70 Polyclonal Antibody
Lane 2: Rabbit Anti-HSP70 Polyclonal Antibody pre-incubated with immunizing peptide
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG

HSP70 Antibody, PAb, Rabbit

ImmunohistochemistryanalysisofhumanLiver tissue slide (Paraffin embedded) using HSP70 Antibody, pAb, Rabbit (GenScript, Cat. No.A01243, 10 ug/ml).

HSP70 Antibody, PAb, Rabbit

ImmunohistochemistryanalysisofhumanSkin tissue slide (Paraffin embedded) using HSP70 Antibody, pAb, Rabbit (GenScript, Cat. No.A01243, 10 ug/ml).

Synonyms Rabbit Anti-HSP70 pAb;
Description HSP70 is a member of the chaperonin family. It stabilizes existing proteins against aggregation and mediates the folding of newly translated polypeptides in both cytosol and organelles. HSP70 participates in all of these processes through its ability to recognize nonnative conformations of other proteins. It binds extended peptide segments with a net hydrophobic character exposed during translation and membrane translocation or following stress-induced damage.
GenScript Rabbit Anti-HSP70 Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 80-130 of human HSP70 protein (Swiss Prot: P08107).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide (KLH-coupled) within residues 80-130 of human HSP70 protein (Swiss Prot: P08107).
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-HSP70 Polyclonal Antibody detects endogenous levels of human HSP70 protein. Sequence homology predicts that it will also react with mouse and rat HSP70 protein.
Species Reactivity Human. This product has not yet been tested with other species.
Predicted Band Size 70 kD
Observed Band Size 70 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volumes of antibody will occasionally become trapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunohistochemistry:5-10 µg/ml
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized

  • HSP70 Antibody, PAb, Rabbit
  • HSP70 Antibody, PAb, Rabbit

    Western blot analysis of Hela cell lysate using 1 µg/ml Rabbit Anti-HSP70 Polyclonal Antibody (GenScript, A01243)
    Lane 1: Rabbit Anti-HSP70 Polyclonal Antibody
    Lane 2: Rabbit Anti-HSP70 Polyclonal Antibody pre-incubated with immunizing peptide
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG

  • HSP70 Antibody, PAb, Rabbit
  • HSP70 Antibody, PAb, Rabbit

    ImmunohistochemistryanalysisofhumanLiver tissue slide (Paraffin embedded) using HSP70 Antibody, pAb, Rabbit (GenScript, Cat. No.A01243, 10 ug/ml).

  • HSP70 Antibody, PAb, Rabbit
  • HSP70 Antibody, PAb, Rabbit

    ImmunohistochemistryanalysisofhumanSkin tissue slide (Paraffin embedded) using HSP70 Antibody, pAb, Rabbit (GenScript, Cat. No.A01243, 10 ug/ml).


Cat. No.
Product Name
Price
A01236-40
HSP70 Antibody, pAb, Rabbit - 40 ug
$99.00
 
Cuadrado I., et al. Labdane diterpenes protect against anoxia/reperfusion injury in cardiomyocytes: involvement of AKT activation. Cell Death Dis. 2011 Nov;2:e229.

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Cat. No. A01243
Size
Price
40 ug $99.00