THE™ HA Tag Antibody, mAb, Mouse
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| Synonyms | Mouse Anti-HA-Tag Monoclonal Antibody; |
| Description | GenScript THETM HA Tag Antibody, mAb, Mouse (A01244) is a highly sensitive monoclonal antibody applicable to HA-tagged fusion protein detection. It belongs to our THETM elite antibody collection. HA, or human influenza hemagglutinin, is a surface glycoprotein required for human influenza virus infectivity. The HA tag does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins. THETM HA Tag Antibody, mAb, Mouse (GenScript, A01244) is a high-affinity monoclonal antibody that can be used to detect HA-tagged proteins. This product detects HA tags derived from an HA molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Immunogen | A KLH coupled synthetic peptide (YPYDVPDYA) |
| Fusion Partner | Spleen cells fused with SP2/0-Ag14 mouse myeloma cells. |
| Purification | Protein A affinity column |
| Subclass | IgG1 |
| Clone ID | 5E11D8 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5mg/ml. |
| Specificity | THETM HA Tag Antibody, mAb, Mouse (A01244) recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml Western blot: 0.1-1.0 µg/ml Immunoprecipitation: 1 µg/ml Immunofluorescent staining: 1 µg/ml Flow cytometry: 1 µg/ml Other applications: user-optimized |
Flow cytometric analysis of non-transfected or HA fusion gene transfected CHO cells using
THETM HA Tag Antibody, mAb, Mouse (GenScript, A01244) (black and pink respectively).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
A01621 |
THE™ HA Tag Antibody [FITC], mAb, Mouse - 100 μg |
$299.00 |
|
A01806-100 |
THE™ HA Tag Antibody [iFluor 488], mAb, Mouse - 100 μg |
$344.00 |
|
A01808-100 |
THE™ HA Tag Antibody [iFluor 647], mAb, Mouse - 100 μg |
$344.00 |
|
A01296 |
THE™ HA Tag Antibody [HRP], mAb, Mouse - 100 μg |
$299.00 |
|
A01244-100 |
THE™ HA Tag Antibody, mAb, Mouse - 100 μg |
$287.00 |
Yi Ma., et al. Linking Ligand Perception By Pepr Pattern Recognition Receptors To Cytosolic Ca2+ Elevation And Downstream Immune Signaling In Plants. Proc Natl Acad Sci U S A. 2012 Nov;109(48):19852-7.
Swaney DL., et al. Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Nat Methods. 2013 Jul;10(7):676-82.
Dasiel O. Borroto-Escuela., et al. Dissecting the conserved NPxxY motif of the M3 muscarinic acetylcholine receptor: critical role of Asp-7.49 for receptor signaling and multiprotein complex formation. Cell Physiol Biochem. 2011 Dec;28(5):1009-12.
Yulia V. Shulga., et al. Phosphatidylinositol-4-Phosphate 5-Kinase Isoforms Exhibit Acyl Chain Selectivity For Both Substrate And Lipid Activator. J Biol Chem. 2012 Oct;287(43):35953 - 35963.
Cheng X., et al. Functional analysis of bovine Nramp1 and production of transgenic cloned embryos in vitro. Zygote. 2013 May;1-10.
Pillay LM., et al. Evaluating the Mutagenic Activity of Targeted Endonucleases Containing a Sharkey FokI Cleavage Domain Variant in Zebrafish. Zebrafish. 2013 Sep;10(3):353-364.
You can visit "Citations Database" for more citations.
Swaney DL., et al. Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Nat Methods. 2013 Jul;10(7):676-82.
Dasiel O. Borroto-Escuela., et al. Dissecting the conserved NPxxY motif of the M3 muscarinic acetylcholine receptor: critical role of Asp-7.49 for receptor signaling and multiprotein complex formation. Cell Physiol Biochem. 2011 Dec;28(5):1009-12.
Yulia V. Shulga., et al. Phosphatidylinositol-4-Phosphate 5-Kinase Isoforms Exhibit Acyl Chain Selectivity For Both Substrate And Lipid Activator. J Biol Chem. 2012 Oct;287(43):35953 - 35963.
Cheng X., et al. Functional analysis of bovine Nramp1 and production of transgenic cloned embryos in vitro. Zygote. 2013 May;1-10.
Pillay LM., et al. Evaluating the Mutagenic Activity of Targeted Endonucleases Containing a Sharkey FokI Cleavage Domain Variant in Zebrafish. Zebrafish. 2013 Sep;10(3):353-364.
You can visit "Citations Database" for more citations.
For more documents, please visit "Technical Support".
| Cat. No. A01244S | ||
|---|---|---|
| Size | Price | |
| 10 μg | $0.00 | |
