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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM HA Tag Antibody, mAb, Mouse

THETM HA Tag Antibody, mAb, Mouse

 THE<sup>TM</sup> HA Tag Antibody, MAb, Mouse

Lane 1-3: 2 μl, 0.4 μl, 0.08 μl N-terminal HA-GST fusion protein in E. coli cell lysate
Lane 4-6: 2 μl, 0.4 μl, 0.08 μl C-terminal HA-GST fusion protein in E. coli cell lysate
Lane 7-9: 500 ng, 250 ng, 125 ng Internal HA tagged Fusion protein (Purified) (GenScript, M0101)
Lane 10: E. coli cell lysate

Primary antibody:
A. 1 μg/ml THETM Anti-HA-Tag Monoclonal Antibody (Mouse) (GenScript, A01244)
B. 1 μg/ml Mouse Anti-HA Monoclonal Antibody (Commerical product, clone 12CA5)

Secondary antibody:
IRDye800 Conjugated affinity Purified Goat Anti-Mouse IgG

 THE<sup>TM</sup> HA Tag Antibody, MAb, Mouse

293 cells transfected with N-terminal HA tag protein
Primary antibody: 1 μg/ml THETM Anti-HA-tag Monoclonal Antibody (Mouse) (GenScript, A01244)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121)

 THE<sup>TM</sup> HA Tag Antibody, MAb, Mouse

293 cells transfected with C-terminal HA tag protein
Primary antibody: 1 μg/ml THETM Anti-HA-tag Monoclonal Antibody (Mouse) (GenScript, A01244)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-mouse IgG (Rockland, 610-102-121)

 THE<sup>TM</sup> HA Tag Antibody, MAb, Mouse

Flow cytometric analysis of non-transfected or HA fusion gene transfected CHO cells using
THETM HA Tag Antibody, mAb, Mouse (GenScript, A01244) (black and pink respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

Synonyms Mouse Anti-HA-Tag Monoclonal Antibody;
Description GenScript THETM HA Tag Antibody, mAb, Mouse (A01244) is a highly sensitive monoclonal antibody applicable to HA-tagged fusion protein detection. It belongs to our THETM elite antibody collection. HA, or human influenza hemagglutinin, is a surface glycoprotein required for human influenza virus infectivity. The HA tag does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins. THETM HA Tag Antibody, mAb, Mouse (GenScript, A01244) is a high-affinity monoclonal antibody that can be used to detect HA-tagged proteins. This product detects HA tags derived from an HA molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors.
Host Species Mouse
Conjugation Unconjugated
Immunogen A KLH coupled synthetic peptide (YPYDVPDYA)
Fusion Partner Spleen cells fused with SP2/0-Ag14 mouse myeloma cells.
Purification Protein A affinity column

Subclass IgG1
Clone ID 5E11D8
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5mg/ml.
Specificity THETM HA Tag Antibody, mAb, Mouse (A01244) recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Immunoprecipitation: 1 µg/ml
Immunofluorescent staining: 1 µg/ml
Flow cytometry: 1 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
IF & ICC Protocol(pdf)

 THE<sup>TM</sup> HA Tag Antibody, MAb, Mouse
 THE<sup>TM</sup> HA Tag Antibody, MAb, Mouse

Lane 1-3: 2 μl, 0.4 μl, 0.08 μl N-terminal HA-GST fusion protein in E. coli cell lysate
Lane 4-6: 2 μl, 0.4 μl, 0.08 μl C-terminal HA-GST fusion protein in E. coli cell lysate
Lane 7-9: 500 ng, 250 ng, 125 ng Internal HA tagged Fusion protein (Purified) (GenScript, M0101)
Lane 10: E. coli cell lysate

Primary antibody:
A. 1 μg/ml THETM Anti-HA-Tag Monoclonal Antibody (Mouse) (GenScript, A01244)
B. 1 μg/ml Mouse Anti-HA Monoclonal Antibody (Commerical product, clone 12CA5)

Secondary antibody:
IRDye800 Conjugated affinity Purified Goat Anti-Mouse IgG

 THE<sup>TM</sup> HA Tag Antibody, MAb, Mouse
 THE<sup>TM</sup> HA Tag Antibody, MAb, Mouse

293 cells transfected with N-terminal HA tag protein
Primary antibody: 1 μg/ml THETM Anti-HA-tag Monoclonal Antibody (Mouse) (GenScript, A01244)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121)

 THE<sup>TM</sup> HA Tag Antibody, MAb, Mouse
 THE<sup>TM</sup> HA Tag Antibody, MAb, Mouse

293 cells transfected with C-terminal HA tag protein
Primary antibody: 1 μg/ml THETM Anti-HA-tag Monoclonal Antibody (Mouse) (GenScript, A01244)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-mouse IgG (Rockland, 610-102-121)

 THE<sup>TM</sup> HA Tag Antibody, MAb, Mouse
 THE<sup>TM</sup> HA Tag Antibody, MAb, Mouse

Flow cytometric analysis of non-transfected or HA fusion gene transfected CHO cells using
THETM HA Tag Antibody, mAb, Mouse (GenScript, A01244) (black and pink respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.


Cat. No.
Product Name
Size
Price
Quantity
A00168-40 HA-tag Antibody, pAb, Goat
40 ug
$99.00
A00169-100 HA-tag Antibody [HRP], pAb, Goat
100 ug
$199.00
A00169-40 HA-tag Antibody [HRP], pAb, Goat
40 ug
$99.00
A00203-40 HA-tag Antibody [Biotin], pAb, Goat
40 ug
$99.00
A01296 THETM HA Tag Antibody [HRP], mAb, Mouse
100 ug
$299.00
A01297 THETM HA Tag Antibody [Biotin], mAb, Mouse
100 ug
$299.00
A01621 THETM HA Tag Antibody [FITC], mAb, Mouse
100 ug
$299.00
A01806-100 THETM HA Tag Antibody [iFluor 488], mAb, Mouse
100 ug
$344.00
A01807-100 THETM HA Tag Antibody [iFluor 555], mAb, Mouse
100 ug
$344.00
A01808-100 THETM HA Tag Antibody [iFluor 647], mAb, Mouse
100 ug
$344.00
 
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Dasiel O. Borroto-Escuela., et al. Dissecting the conserved NPxxY motif of the M3 muscarinic acetylcholine receptor: critical role of Asp-7.49 for receptor signaling and multiprotein complex formation. Cell Physiol Biochem. 2011 Dec;28(5):1009-12.

Swaney DL., et al. Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Nat Methods. 2013 Jul;10(7):676-82.

Yi Ma., et al. Linking Ligand Perception By Pepr Pattern Recognition Receptors To Cytosolic Ca2+ Elevation And Downstream Immune Signaling In Plants. Proc Natl Acad Sci U S A. 2012 Nov;109(48):19852-7.

Y Liu, et al. Modulation of hepatitis B surface antigen secretion by annexin II expressed in hepatitis B virus‑producing hepatoma cells. Mol Med Rep. 2014Dec.;10(6):3113-7

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Cat. No. A01244
Size
Price
100 ug $287.00
 
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