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Home » Gene Synthesis & Molecular Biology Services » Geminin Antibody, pAb, Rabbit

Geminin Antibody, pAb, Rabbit

Geminin Antibody, PAb, Rabbit

Western blot analysis of cell lysates using Geminin Antibody, pAb, Rabbit (GenScript, A01251, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 24 kD
Observed Size: 24 kD

Geminin Antibody, PAb, Rabbit

Flow cytometric analysis of HeLa cells using Geminin Antibody, pAb, Rabbit (GenScript, A01251; shaded histogram) or with an isotype
control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

Geminin Antibody, PAb, Rabbit

Western blot analysis of human recombinant Geminin fusion protein using Geminin Antibody, pAb, Rabbit (GenScript, A01251, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 38 kD
Observed Size: 38 kD

Geminin Antibody, PAb, Rabbit

Immunohistochemistry analysis of human testis tissue slide (Paraffin embedded) using Geminin Antibody, pAb, Rabbit (GenScript, Cat.No. A01251, 10 µg/ml)

Synonyms Rabbit Anti-Geminin pAb;
Description Geminin inhibits DNA replication by preventing the incorporation of the MCM complex into the prereplication complex (pre-RC). It is absent during the G1 phase, accumulates during the S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. Geminin contains 212 amino acids and has a destruction box sequence of RRTLKVIQP. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

GenScript Geminin Antibody, pAb, Rabbit is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 50-100 of human geminin protein (Swiss Prot: O75496).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide within residues 50-100 of human geminin protein (Swiss Prot: O75496).
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity GenScript Geminin Antibody, pAb, Rabbit detects endogenous levels of human geminin protein.
Species Reactivity Human. This product has not been tested with other species yet.
Predicted Band Size 24 kD
Observed Band Size 24 kD, 38 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Immunohistochemistry: 5-10 µg/ml
Other applications: user-optimized

  • Geminin Antibody, PAb, Rabbit
  • Geminin Antibody, PAb, Rabbit

    Western blot analysis of cell lysates using Geminin Antibody, pAb, Rabbit (GenScript, A01251, 1 µg/ml)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 24 kD
    Observed Size: 24 kD

  • Geminin Antibody, PAb, Rabbit
  • Geminin Antibody, PAb, Rabbit

    Flow cytometric analysis of HeLa cells using Geminin Antibody, pAb, Rabbit (GenScript, A01251; shaded histogram) or with an isotype
    control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

  • Geminin Antibody, PAb, Rabbit
  • Geminin Antibody, PAb, Rabbit

    Western blot analysis of human recombinant Geminin fusion protein using Geminin Antibody, pAb, Rabbit (GenScript, A01251, 1 µg/ml)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 38 kD
    Observed Size: 38 kD

  • Geminin Antibody, PAb, Rabbit
  • Geminin Antibody, PAb, Rabbit

    Immunohistochemistry analysis of human testis tissue slide (Paraffin embedded) using Geminin Antibody, pAb, Rabbit (GenScript, Cat.No. A01251, 10 µg/ml)


Wang W., et al. Emi1 preferentially inhibits ubiquitin chain elongation by the anaphase-promoting complex. Nat Cell Biol. 2013 Jul;15(7):797-806.

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Cat. No. A01251
Size
Price
40 ug $99.00