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Catalog Products » Antibodies » Primary Antibodies » GM-CSF Antibody (1B3E11), mAb, Mouse

GM-CSF Antibody (1B3E11), mAb, Mouse

Synonyms Mouse Anti-huGM-CSF mAb;
Description Granulocyte-Macrophage-CSF induces myeloid progenitor cells from bone marrow to form colonies containing macrophages and granulocytes in a semisolid media. GM-CSF also acts upon mature macrophages, eosinophils and neutrophils to stimulate various functional activities. GM-CSF is an acidic glycoprotein (23 kD mouse, 18-22 kD human) which binds to high affinity receptors on GM-CSF sensitive cells. Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific. Other growth factors and CSFs modulate receptor binding or actions of GM-CSF.
Host Species Mouse
Antigen Species Human
Conjugation Unconjugated
Immunogen GM-CSF full length recombinant protein
Fusion Partner Spleen cells fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A affinity column

Subclass Mouse IgG2b, k
Clone ID 1B3E11
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity This product reacts with human GM-CSF. It has not yet been tested with other species.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

  • GM-CSF Antibody (1B3E11), MAb, Mouse
  • GM-CSF Antibody (1B3E11), MAb, Mouse

    Western blot analysis of Mouse Anti-huGM-CSF Monoclonal Antibody (GenScript, A01287)
    Loading: GM-CSF fusion protein
    Primary antibody: 1 µg/ml Mouse Anti-huGM-CSF Monoclonal Antibody (GenScript, A01287)
    Secondary antibody: IRDyeTM 800 Conjugated affinity Purified anti-Mouse IgG [Goat]




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Cat. No. A01287
Size Price
40 ug $99.00