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Catalog Products » Antibodies » Epitope Tag Antibodies » THETM HA Tag Antibody [Biotin], mAb, Mouse

THETM HA Tag Antibody [Biotin], mAb, Mouse

Synonyms Mouse Anti-HA-tag [Biotin] mAb;
Description GenScript THETM HA Tag Antibody [Biotin], mAb, Mouse is the biotin conjugated THETM HA Tag Antibody, mAb, Mouse (A01244) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of HA in fusion proteins.
Host Species Mouse
Antigen Species Human
Conjugation Biotin
Immunogen A synthetic peptide (YPYDVPDYA) coupled to KLH
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A affinity column

Subclass IgG1, k
Clone ID 5E11D8
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity THETM HA Tag Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal and internal HA tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05 -0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized

  •  THE<sup>TM</sup> HA Tag Antibody [Biotin], MAb, Mouse
  •  THE<sup>TM</sup> HA Tag Antibody [Biotin], MAb, Mouse

    Western blot analysis of Multiple Tag (GenScript, M0101) using THETM HA Tag Antibody [Biotin], mAb, Mouse (GenScript, A01297, 1 µg/ml)
    The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
    Predicted Size: 52 kD
    Observed Size: 52 kD

Cat. No.
Product Name
THETM HA Tag Antibody [iFluor 647], mAb, Mouse - 100 ug
THETM HA Tag Antibody [iFluor 555], mAb, Mouse - 100 ug
THETM HA Tag Antibody [iFluor 488], mAb, Mouse - 100 ug
THETM HA Tag Antibody [FITC], mAb, Mouse - 100 ug
THETM HA Tag Antibody [HRP], mAb, Mouse - 100 ug
THETM HA Tag Antibody, mAb, Mouse - 100 ug
HA-tag Antibody [HRP], pAb, Goat - 100 ug
HA-tag Antibody [HRP], pAb, Goat - 40 ug
HA-tag Antibody, pAb, Goat - 40 ug

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Cat. No. A01297
100 ug $299.00