THETM HA Tag Antibody [Biotin], mAb, Mouse Antibody

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Overview

Synonyms	Mouse Anti-HA-tag [Biotin] mAb;
Description	GenScript THE^TM HA Tag Antibody [Biotin], mAb, Mouse is the biotin conjugated THE^TM HA Tag Antibody, mAb, Mouse (A01244) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of HA in fusion proteins.
Host Species	Mouse
Antigen Species	Human
Conjugation	Biotin
Immunogen	A synthetic peptide (YPYDVPDYA) coupled to KLH
Fusion Partner	Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification	Protein A affinity column

Properties

Subclass	IgG1, k
Clone ID	5E11D8
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity	THE^TM HA Tag Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal and internal HA tagged fusion proteins.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05 -0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized

Technical Manual

ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Examples

THE<sup>TM</sup> HA Tag Antibody [Biotin], MAb, Mouse

Western blot analysis of Multiple Tag (GenScript, M0101) using THE^TM HA Tag Antibody [Biotin], mAb, Mouse (GenScript, A01297, 1 µg/ml)
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensor^TM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

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