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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM HA Tag Antibody [Biotin], mAb, Mouse

THETM HA Tag Antibody [Biotin], mAb, Mouse

Synonyms Mouse Anti-HA-tag [Biotin] mAb;
Description GenScript THETM HA Tag Antibody [Biotin], mAb, Mouse is the biotin conjugated THETM HA Tag Antibody, mAb, Mouse (A01244) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of HA in fusion proteins.
Host Species Mouse
Antigen Species Human
Conjugation Biotin
Immunogen A synthetic peptide (YPYDVPDYA) coupled to KLH
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A affinity column

Subclass IgG1, k
Clone ID 5E11D8
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity THETM HA Tag Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal and internal HA tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05 -0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

 THE<sup>TM</sup> HA Tag Antibody [Biotin], MAb, Mouse
 THE<sup>TM</sup> HA Tag Antibody [Biotin], MAb, Mouse

Western blot analysis of Multiple Tag (GenScript, M0101) using THETM HA Tag Antibody [Biotin], mAb, Mouse (GenScript, A01297, 1 µg/ml)
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD


Cat. No.
Product Name
Size
Price
Quantity
A00168-40 HA-tag Antibody, pAb, Goat
40 ug
$99.00
A00169-100 HA-tag Antibody [HRP], pAb, Goat
100 ug
$199.00
A00169-40 HA-tag Antibody [HRP], pAb, Goat
40 ug
$99.00
A00203-40 HA-tag Antibody [Biotin], pAb, Goat
40 ug
$99.00
A01244-100 THETM HA Tag Antibody, mAb, Mouse
100 ug
$287.00
A01296 THETM HA Tag Antibody [HRP], mAb, Mouse
100 ug
$299.00
A01621 THETM HA Tag Antibody [FITC], mAb, Mouse
100 ug
$299.00
A01806-100 THETM HA Tag Antibody [iFluor 488], mAb, Mouse
100 ug
$344.00
A01807-100 THETM HA Tag Antibody [iFluor 555], mAb, Mouse
100 ug
$344.00
A01808-100 THETM HA Tag Antibody [iFluor 647], mAb, Mouse
100 ug
$344.00
 


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Cat. No. A01297
Size
Price
100 ug $299.00
24 hour
 
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