THETM HA Tag Antibody [Biotin], mAb, Mouse
| Synonyms | Mouse Anti-HA-tag [Biotin] mAb; |
| Description | GenScript THETM HA Tag Antibody [Biotin], mAb, Mouse is the biotin conjugated THETM HA Tag Antibody, mAb, Mouse (A01244) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of HA in fusion proteins. |
| Host Species | Mouse |
| Antigen Species | Human |
| Conjugation | Biotin |
| Immunogen | A synthetic peptide (YPYDVPDYA) coupled to KLH |
| Fusion Partner | Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells |
| Purification | Protein A affinity column |
| Subclass | IgG1, k |
| Clone ID | 5E11D8 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
| Specificity | THETM HA Tag Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal and internal HA tagged fusion proteins. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05 -0.2 µg/ml Western blot: 0.5-1 µg/ml Other applications: user-optimized |
![THE<sup>TM</sup> HA Tag Antibody [Biotin], MAb, Mouse](/product/documents/down?doc_name=9448_20090223022402.JPG&file=filecenter/document/9448_20090223022402.JPG "THE<sup>TM</sup> HA Tag Antibody [Biotin], MAb, Mouse")
Western blot analysis of Multiple Tag (GenScript, M0101) using THETM HA Tag Antibody [Biotin], mAb, Mouse (GenScript, A01297, 1 µg/ml)
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD
Cat. No. |
Product Name |
Price |
|
|---|---|---|---|
A01808-100 |
THETM HA Tag Antibody [iFluor 647], mAb, Mouse - 100 ug |
$344.00 |
|
A01806-100 |
THETM HA Tag Antibody [iFluor 488], mAb, Mouse - 100 ug |
$344.00 |
|
A01621 |
THETM HA Tag Antibody [FITC], mAb, Mouse - 100 ug |
$299.00 |
|
A01296 |
THETM HA Tag Antibody [HRP], mAb, Mouse - 100 ug |
$299.00 |
|
A01244-100 |
THETM HA Tag Antibody, mAb, Mouse - 100 ug |
$287.00 |
|
A00169-40 |
HA-tag Antibody [HRP], pAb, Goat - 40 ug |
$99.00 |
|
A00168-40 |
HA-tag Antibody, pAb, Goat - 40 ug |
$99.00 |
For more documents, please visit "Technical Support".
| Cat. No. A01297 | ||
|---|---|---|
Size |
Price |
|
| 100 ug | $299.00 | |
