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Synaptophysin Antibody, pAb, Rabbit

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Immunohistochemistry analysis of human brain tissue slide (Paraffin embedded) using Rabbit Anti-Synaptophysin Polyclonal Antibody (Left, GenScript, A01307)
and Purified Rabbit IgG (Whole molecule) Control (Right, GenScript, A01008)

Western blot analysis of tissue lysates using 1 µg/ml Rabbit Anti-Synaptophysin Polyclonal Antibody (GenScript, A01307)

The signal was developed with IRDye^TM 800 Conjugated Goat Anti-Rabbit IgG.

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Overview

Synonyms	Rabbit Anti Synaptophysin pAb;
Description	Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms. The protein resides in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. Synaptophysin is possibly involved in structural functions by organizing other membrane components or in targeting the vesicles to the plasma membrane. GenScript Rabbit Anti-Synaptophysin Polyclonal Antibody is developed in Rabbit using a KLH-coupled synthetic peptide from within residues 200-250 of human Synaptophysin protein (Swiss Prot: P08247).
Host Species	Rabbit
Antigen Species	Human
Conjugation	Unconjugated
Immunogen	KLH-coupled synthetic peptide from within residues 200-250 of human Synaptophysin protein (Swiss Prot: P08247).
Purification	Immunoaffinity chromatography

Properties

Subclass	Rabbit IgG
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity	GenScript Rabbit Anti-Synaptophysin Polyclonal Antibody detects endogenous levels of human, mouse and rat Synaptophysin protein.
Species Reactivity	Human, mouse, and rat
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Immunohistochemistry: 5-10 µg/ml
Immunoprecipitation: 2-10 µg/mg of lysate
Other Applications: user-optimized

Examples

Western blot analysis of tissue lysates using 1 µg/ml Rabbit Anti-Synaptophysin Polyclonal Antibody (GenScript, A01307)

The signal was developed with IRDye^TM 800 Conjugated Goat Anti-Rabbit IgG.