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Catalog Products » Antibodies » Primary Antibodies » GPCR Antibodies » ADP Antibody, pAb, Rabbit

ADP Antibody, pAb, Rabbit

Synonyms Rabbit Anti-ADP pAb;
Description Adenosine diphosphate, abbreviated ADP, is the product of adenosine triphosphate (ATP) dephosphorylation by ATPases. As a coenzyme,ADP is an important intermediate in cellular metabolism as the partially dephosphorylated form of ATP. The compound is 5-adenylic acid with an additional phosphate group attached through a pyrophosphate bond. ADP is produced from ATP and can be reconverted to ATP in coupled reactions concerned with the energy metabolism of living systems. ADP is also produced from 5-adenylic acid by the transfer of a phosphate group from adenosine triphosphate in a reaction that is catalyzed by an enzyme, myokinase.

GenScript Rabbit Anti-ADP Polyclonal Antibody is developed in rabbit using ADP-6-KLH as the immunogen. The antibody can be used to develop assays for kinase and ATPase activity assessment.
Host Species Rabbit
Conjugation Unconjugated
Immunogen ADP-6-KLH
Purification Antiserum

Subclass Rabbit IgG
Appearance Lyophilized form
Concentration GenScript Rabbit Anti-ADP Polyclonal Antibody is lyophilized from 40 μl or 200 μl of antiserum with 0.02% of sodium azide as preservative before lyophilization.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final volume of 40 μl or 200 μl.
Specificity The specificity of the antiserum is defined as the ratio of antigen concentration to cross-reactant concentration at 50% inhibition of maximum binding. The cross-reactivity data obtained in competitive ELISA system is as follows:

Compound% Cross-reactivity
ADP100
GDP<0.0787
AMP<0.0001
GMP<0.0001
ATP<2.7200
GTP<0.0030
cAMP<0.0001
cGMP<0.0001
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 1:5,000-1:30,000

  • ADP Antibody, PAb, Rabbit
  • ADP Antibody, PAb, Rabbit

    Competitive ELISA of ADP standard curve using
    Rabbit Anti-ADP Polyclonal Antibody (GenScript, A01316)


Cat. No.
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A01819-100
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A01818-100
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A01817-100
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A01799-40
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A01799-100
THETM ADP Antibody, mAb, Mouse - 100 ug
$350.00
 
Xie L., et al. Drug discovery using chemical systems biology: weak inhibition of multiple kinases may contribute to the anti-cancer effect of nelfinavir. PLoS Biol. 2011 Apr;7(4):e1002037.

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Cat. No. A01316
Size
Price
40 μl $99.00
200 μl $349.00