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Catalog Products » Antibodies » Secondary Antibodies » Goat Anti-Mouse IgG Antibody (H&L) [FITC], pAb

Goat Anti-Mouse IgG Antibody (H&L) [FITC], pAb

Synonyms Goat Anti-Mouse IgG (H&L) [FITC] pAb;
Description Goat Anti-Mouse IgG (H&L) [FITC] Polyclonal Antibody is developed in goat using purified mouse IgG whole molecule. This polyclonal antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to FITC.

Goat Anti-Mouse IgG (H&L) [FITC] Polyclonal Antibody is suitable for immunocytochemistry, immunohistochemistry and flow cytometry analysis as well as other antibody based fluorescent assays.
Absorption Wavelength: 492 nm
Emission Wavelength: 518 nm
Fluorochrome/Protein Ratio: >3 moles FITC per mole of Goat IgG.
Host Species Goat
Antigen Species Mouse
Conjugation Fluorescein (FITC)
Immunogen Purified mouse IgG whole molecule
Purification Immunoaffinity chromatography
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Subclass Goat IgG
Concentration 1 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with 1 ml of deionized water (or equivalent) to an antibody concentration of 1 mg/ml.
Specificity This antibody reacts with mouse IgG heavy and light chains.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by primary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Flow cytometry: 1:200-1:500
Immunocytochemistry: 1:200-1:500
Immunohistochemistry: 1:100-1:300
Other Applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)


Cat. No.
Product Name
Price
A00160
Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb - 1 mg
$79.00
 
Li XH., et al. Expression of Trichoderma viride endoglucanase III in the larvae of silkworm, Bombyx mori L. and characteristic analysis of the recombinant protein. Mol Biol Rep. 2011 Aug;38(6):3897-3902.

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Cat. No. A01318
Size
Price
1 mg $79.00
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