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Home » Gene Synthesis & Molecular Biology Services » Neurofascin Antibody, pAb, Rabbit

Neurofascin Antibody, pAb, Rabbit

Neurofascin Antibody, PAb, Rabbit

Western blot analysis of tissue lysates using 1 µg/ml Rabbit Anti-Neurofascin Polyclonal Antibody (GenScript, A01377)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 150 KD
Observed Size: 150 KD

Neurofascin Antibody, PAb, Rabbit

ImmunohistochemistryanalysisofhumanBrain, Cortex tissue slide (Paraffin embedded) using Neurofascin Antibody, pAb, Rabbit (GenScript, Cat. No.A01377, 10 ug/ml).

Synonyms Rabbit Anti-Neurofascin pAb;
Description Neurofascin is one member of Li family which involves vertebrate L1, Ng-CAM, and Nr-CAM. Neurofascin protein is a nervous system cell adhesion molecule that contains six Ig domains and multiple fibronectin type III repeats in its extracellular region. Expression level of neurofascin is abundant in the nervous system during early development as well as in adult stage. Phosphorylation of the FIGQY tyrosine of neurofascin abolishes ankyrin binding and increases lateral mobility of neurofascin. Phospho-FIGQY neurofascin associates with doublecortin, which probably plays an important role in neuronal migration.

GenScript Rabbit Anti-Neurofascin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from amino terminal of human Neurofascin. (Swiss Prot: O94856).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide from amino terminal of human Neurofascin (Swiss Prot: O94856).
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-Neurofascin Polyclonal Antibody detects endogenous levels of rat and mouse Neurofascin protein. It is predicted to react with human neurofascin protein according to sequence homology.
Species Reactivity Rat and mouse. This product has not been tested with other species yet.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 1-2 µg/ml

Immunohistochemistry:5-10 µg/ml Other Applications: user-optimized

  • Neurofascin Antibody, PAb, Rabbit
  • Neurofascin Antibody, PAb, Rabbit

    Western blot analysis of tissue lysates using 1 µg/ml Rabbit Anti-Neurofascin Polyclonal Antibody (GenScript, A01377)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 150 KD
    Observed Size: 150 KD

  • Neurofascin Antibody, PAb, Rabbit
  • Neurofascin Antibody, PAb, Rabbit

    ImmunohistochemistryanalysisofhumanBrain, Cortex tissue slide (Paraffin embedded) using Neurofascin Antibody, pAb, Rabbit (GenScript, Cat. No.A01377, 10 ug/ml).




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Cat. No. A01377
Size
Price
40 ug $99.00