Catalog Products » Antibodies » Epitope Tag Antibodies » GST-tag Antibody, pAb, Goat

GST-tag Antibody, pAb, Goat

*This product has been discontinued!*
Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogeneity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be used in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.GenScript Goat Anti-GST-tag Polyclonal Antibody is developed in goat using a recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum.
A01379
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Overview
Specificity GenScript Goat Anti-GST-tag Polyclonal Antibody detects GST fusion proteins in almost expression systems including bacterial expression system, mammalian expression system and other systems.
Host Species Goat
Immunogen Recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum.

Properties
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Purification Immunoaffinity chromatography

Background
Target Background Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogeneity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be used in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.

GenScript Goat Anti-GST-tag Polyclonal Antibody is developed in goat using a recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum.
Synonyms Goat Anti-GST pAb;

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