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Catalog Products » Other Products » Catalog Peptides » All Catalog Peptides » MEP50 Antibody, pAb, Rabbit

MEP50 Antibody, pAb, Rabbit

MEP50 Antibody, PAb, Rabbit

Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-MEP50 Polyclonal Antibody (GenScript, A01385)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 37 KD
Observed Size: 40 KD

MEP50 Antibody, PAb, Rabbit

ImmunohistochemistryanalysisofhumanPancreas tissue slide (Paraffin embedded) using MEP50 Antibody, pAb, Rabbit (GenScript, Cat. No.A01385, 10 ug/ml).

MEP50 Antibody, PAb, Rabbit

ImmunohistochemistryanalysisofhumanSpleen tissue slide (Paraffin embedded) using MEP50 Antibody, pAb, Rabbit (GenScript, Cat. No.A01385, 10 ug/ml).

Synonyms Rabbit Anti-MEP50 pAb;
Description MEP50 (methylosome protein 50), also known as WDR77, is a component of protein arginine methyltransferase complex containing at least PRMT5, CLNS1A and MEP50. It binds histone H2A selectively among core histones, and mediates transcriptional repression of protein arginine methyltransferase PRMT5. MEP50 is a WD repeat protein which interacts with an arginine protein JBP1. MEP50 plays a critical role in methylosome activity and may regulate the transfer of Sm proteins to the SMN (survival of motor neurons) complex, an early step in the assembly of U snRNPs. In addition, MEP50 may function to mediate the interaction of multiple substrates in the methylosome.

GenScript Rabbit Anti-MEP50 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from carboxyl terminal of human MEP50. (Swiss Prot: Q9BQA1).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide from carboxyl terminal of human MEP50 (Swiss Prot: Q9BQA1)
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-MEP50 Polyclonal Antibody detects endogenous levels of human MEP50 protein. It is predicted to react with sumatran orangutan MEP50 protein according to sequence homology.
Species Reactivity Human. This product has not been tested with other species yet.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 1-2 µg/ml

Immunohistochemistry:5-10 µg/ml Other Applications: user-optimized

  • MEP50 Antibody, PAb, Rabbit
  • MEP50 Antibody, PAb, Rabbit

    Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-MEP50 Polyclonal Antibody (GenScript, A01385)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 37 KD
    Observed Size: 40 KD

  • MEP50 Antibody, PAb, Rabbit
  • MEP50 Antibody, PAb, Rabbit

    ImmunohistochemistryanalysisofhumanPancreas tissue slide (Paraffin embedded) using MEP50 Antibody, pAb, Rabbit (GenScript, Cat. No.A01385, 10 ug/ml).

  • MEP50 Antibody, PAb, Rabbit
  • MEP50 Antibody, PAb, Rabbit

    ImmunohistochemistryanalysisofhumanSpleen tissue slide (Paraffin embedded) using MEP50 Antibody, pAb, Rabbit (GenScript, Cat. No.A01385, 10 ug/ml).




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Cat. No. A01385
Size
Price
40 ug $99.00