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Catalog Products » Antibodies » Primary Antibodies » Alzheimer's Disease Antibody list » Tau Antibody (Ser396), pAb, Rabbit

Tau Antibody (Ser396), pAb, Rabbit

*This product has been discontinued!*
Specificity GenScript Rabbit Anti-Tau (Ser396) Polyclonal Antibody detects endogenous levels of human, mouse, and rat tau protein.
Host Species Rabbit
Immunogen KLH-coupled synthetic peptide corresponding to residues surrounding serine 396 of human tau
Species Reactivity Human, mouse, and rat. This product has not been tested with other species yet.
Conjugate Unconjugated

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.5-1 µg/ml
Other applications User-optimized

Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable

Target Background Tau is a microtubule-associated phosphoprotein (MAP) localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms, ranging from 352 to 441 amino acids. The tau variants differ from each other with the presence of either three or four repeat-regions near the carboxy-terminal and the absence or presence of one or two inserts near the amino-terminal.Tau is hyperphosphorylated by ERK, GSK-3β, TPKII, and CDK5. At least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3β transfection phosphorylates serines 199, 202, 235, 396, 404, 413, and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of tau. Phosphorylation on these sites reduces the ability of a given tau species to promote microtubule self-assembly. Hyperphosphorylated tau is the major protein found in the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimers disease (AD). The PHFs are also found in the lesions of other central nervous system disorders.GenScript Rabbit Anti-Tau (Ser396) Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide corresponding to residues surrounding serine 396 of human tau.
Synonyms Rabbit Anti-Tau (Ser396) pAb;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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Cat. No. A01387