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Resources » Research Applications » Stem Cell Research » Stem Cell Antibodies » NSE Antibody, pAb, Rabbit

NSE Antibody, pAb, Rabbit

NSE Antibody, PAb, Rabbit

Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-NSE Polyclonal Antibody (GenScript, A01405)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 47 KD
Observed Size: 47 KD

NSE Antibody, PAb, Rabbit

Western blot analysis of cell and tissue lysates using 1 µg/ml Rabbit Anti-NSE Polyclonal Antibody (GenScript, A01405)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 47 KD
Observed Size: 47 KD.

NSE Antibody, PAb, Rabbit

Immunohistochemistry analysis of human brain tissue slide (Paraffin embedded) using Rabbit Anti-NSE Polyclonal Antibody (Left, GenScript, A01405)
and Purified Rabbit IgG (Whole molecule) Control (Right, GenScript, A01008)

Synonyms Rabbit Anti-NSE pAb;
Description Enolase is a glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase has three subunits (alpha, beta and gamma) that can form homo and heterodimers. Homodimers of gamma enolase are neuronal-specific. NSE (neuron specific enolase) is expressed primarily in neurons. It is also found in elevated levels in plasma in certain neoplasias including pediatric neuroblastoma and small cell lung cancer. NSE is widely used as a diagnostic marker in a variety of clinical assays.

GenScript Rabbit Anti-NSE Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human NSE (Swiss Prot: P09104).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide from N-terminal of human NSE (Swiss Prot: P09104).
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-NSE Polyclonal Antibody detects endogenous levels of human, mouse, and rat NSE protein. It may cross-reacts with beta enolase according to sequence homology.
Species Reactivity Human, mouse, and rat. This product has not been tested with other species yet.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Immunohistochemistry: 5-10 µg/ml
Other Applications: user-optimized

  • NSE Antibody, PAb, Rabbit
  • NSE Antibody, PAb, Rabbit

    Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-NSE Polyclonal Antibody (GenScript, A01405)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 47 KD
    Observed Size: 47 KD

  • NSE Antibody, PAb, Rabbit
  • NSE Antibody, PAb, Rabbit

    Western blot analysis of cell and tissue lysates using 1 µg/ml Rabbit Anti-NSE Polyclonal Antibody (GenScript, A01405)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 47 KD
    Observed Size: 47 KD.

  • NSE Antibody, PAb, Rabbit
  • NSE Antibody, PAb, Rabbit

    Immunohistochemistry analysis of human brain tissue slide (Paraffin embedded) using Rabbit Anti-NSE Polyclonal Antibody (Left, GenScript, A01405)
    and Purified Rabbit IgG (Whole molecule) Control (Right, GenScript, A01008)


Cat. No.
Product Name
Price
A01406-40
NSE Antibody, pAb, Rabbit - 40 ug
$49.5
$99.00
 


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Cat. No. A01405
Size
Price
40 ug $49.5
$99.00