• English
  • Sign In
  • Contact Us
×
Resources » Research Applications » Stem Cell Research » Stem Cell Antibodies » Myelin Basic Protein Antibody, pAb, Rabbit

Myelin Basic Protein Antibody, pAb, Rabbit

Synonyms Rabbit Anti-Myelin Basic Protein pAb;
Description Myelin basic protein (MBP) participates in the process of myelination and has a role in both its formation and stabilization. Alternative splice combined with post-translational modifications give rise to a wide spectrum of isomers, with each of them potentially having a particular function. The MBP classic group isoforms (isoforms 4 to 14) are the most abundant protein components of the myelin membrane in CNS. MBP phosphorylation at threonine 125 modulates the contribution of MBP to the stability of the myelin sheath. Myelin basic protein serves as an oligodendrocyte marker in neuroscience study.

GenScript Rabbit Anti-Myelin Basic Protein Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human Myelin Basic Protein (Swiss Prot: P02686).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide from C-terminal of human Myelin Basic Protein (Swiss Prot: P02686).
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-Myelin Basic Protein Polyclonal Antibody detects endogenous levels of mouse and rat Myelin Basic Protein. It is predicted to react with human Myelin Basic Protein according to sequence homology.
Species Reactivity Mouse and rat. This product has not been tested with other species yet.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other Applications: user-optimized.

  • Myelin Basic Protein Antibody, PAb, Rabbit
  • Myelin Basic Protein Antibody, PAb, Rabbit

    Western blot analysis of tissue lysates using 1 µg/ml Rabbit Anti-Myelin Basic Protein Polyclonal Antibody (GenScript, A01407)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 14-33 KD
    Observed Size: 14-33 KD


Kraitsy K, et al. Repetitive long-term hyperbaric oxygen treatment (HBOT) administered after experimental traumatic brain injury in rats induces significant remyelination and a recovery of sensorimotor function. PLoS One. 2014

You can visit "Citations Database" for more citations.






For more documents, please visit "Technical Support".

Cat. No. A01407
Size
Price
40 ug $99.00