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Support » Promotions » Product Promotions » Free Samples » THETM alpha Tubulin Antibody, mAb, Mouse

THETM alpha Tubulin Antibody, mAb, Mouse

Synonyms α-tubulin mAb; alpha tubulin mAb
Description Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Microtubules are obligate proteinaceous elements found in nearly all eukaryotic cells. The availability of monoclonal antibodies to α-tubulin uses as a common housekeeping protein and serves as a specific and useful tool in studying proper interpretation of western blots.
Host Species Mouse
Conjugation Unconjugated
Immunogen A synthetic peptide (ALEKDYEEVGVDSVE) coupled to KLH
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A affinity column

Subclass IgG2b,κ
Clone ID 1F4E3
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity THETM alpha Tubulin Antibody, mAb, Mouse reacts with mouse, human, hamster, monkey, and pig α-tubulin. This product has not yet been tested in other species.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Immunofluorescent staining: 1 µg/ml
Other applications: user-optimized

Cat. No. Product Name Quantity Price
THETM alpha Tubulin Antibody [Biotin], mAb, Mouse - 100 ug
THETM alpha Tubulin Antibody [HRP], mAb, Mouse - 100 ug
THETM alpha Tubulin Antibody, mAb, Mouse - 100 ug
Liberman R., et al. Regulated Assembly of the V-ATPase is Increased During Cluster Disruption-Induced Maturation of Dendritic Cells Through a PI-3 Kinase/mTOR-dependent Pathway. J Biol Chem. 2014 Jan;289(3):1355-63.

Lakshmana MK., et al. Role of RanBP9 on amyloidogenic processing of APP and synaptic protein levels in the mouse brain. FASEB J. 2012 May;26(5):2072-83.

Schmid M., et al. Apicomplexan Parasite, Eimeria falciformis, Co-opts Host Tryptophan Catabolism for Life Cycle Progression in Mouse. J Biol Chem. 2012 Jun;287(24):20197-207.

Amita M., et al. Complete and unidirectional conversion of human embryonic stem cells to trophoblast by BMP4. Proc Natl Acad Sci U S A. 2013 Mar;110(13):E1212 - E1221.

Chen HW., et al. Inhibitory and combinatorial effect of diphyllin, a v-ATPase blocker, on influenza viruses. Antiviral Res. 2013 Sep;99(3):371-82.

Kuo HP., et al. Epigenetic Roles of MLL Oncoproteins Are Dependent on NF-κB. Cancer Cell. 2013 Oct;24(4):423-37

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Cat. No. A01410S
Size Price
10 ug $0.00