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THETM alpha Tubulin Antibody, mAb, Mouse
THETM alpha Tubulin Antibody, mAb, Mouse
Comparison of THETM alpha Tubulin Antibody, mAb, Mouse (GenScript, A01410) with Mouse Anti-alpha Tubulin mAb (Competitor A) by Western blot.
A: THETM alpha Tubulin Antibody, mAb, Mouse (GenScript, A01410, 1 µg/ml)
B: Mouse Anti-alpha Tubulin Monoclonal Antibody (Clone: DM1A, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Predicted Size: 50 kD
Observed Size: 50 kD
Western blot analysis of cell and tissue lysates using THETM alpha Tubulin Antibody, mAb, Mouse (GenScript, A01410, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Predicted Size: 50 kD
Observed Size: 50 kD
Immunofluorescence analysis of 293 cells
Primay antibody: 1 μg/ml THETM Anti-alpha tubulin Monoclonal Antibody (Mouse) (GenScript, A01410)
Secondary antibody: Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (H&L) (Goat) (Rockland, 1:1,000, 610-102-121)
| Synonyms | α-tubulin mAb; |
| Description | Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Microtubules are obligate proteinaceous elements found in nearly all eukaryotic cells. The availability of monoclonal antibodies to α-tubulin uses as a common housekeeping protein and serves as a specific and useful tool in studying proper interpretation of western blots. |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide (ALEKDYEEVGVDSVE) coupled to KLH |
| Fusion Partner | Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells |
| Purification | Protein A affinity column |
| Subclass | IgG2b,κ |
| Clone ID | 1F4E3 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
| Specificity | THETM alpha Tubulin Antibody, mAb, Mouse reacts with mouse, human, hamster, monkey, and pig α-tubulin. This product has not yet been tested in other species. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Note | GenScript can offer this product per customer's reuqest including product size, buffer components,etc. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western blot: 0.1-1.0 µg/ml Immunofluorescent staining: 1 µg/ml Other applications: user-optimized |
Comparison of THETM alpha Tubulin Antibody, mAb, Mouse (GenScript, A01410) with Mouse Anti-alpha Tubulin mAb (Competitor A) by Western blot.
A: THETM alpha Tubulin Antibody, mAb, Mouse (GenScript, A01410, 1 µg/ml)
B: Mouse Anti-alpha Tubulin Monoclonal Antibody (Clone: DM1A, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Predicted Size: 50 kD
Observed Size: 50 kD
Western blot analysis of cell and tissue lysates using THETM alpha Tubulin Antibody, mAb, Mouse (GenScript, A01410, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Predicted Size: 50 kD
Observed Size: 50 kD
Immunofluorescence analysis of 293 cells
Primay antibody: 1 μg/ml THETM Anti-alpha tubulin Monoclonal Antibody (Mouse) (GenScript, A01410)
Secondary antibody: Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (H&L) (Goat) (Rockland, 1:1,000, 610-102-121)
Cat. No. |
Product Name |
Price |
|
|---|---|---|---|
A01717-40 |
β-tubulin Antibody (2G7D4), mAb, Mouse - 40 ug |
$99.00 |
|
A01627-40 |
Neuron specific β-Tubulin III Antibody, pAb, Rabbit - 40 ug |
$99.00 |
|
A01545 |
THETM alpha Tubulin Antibody [Biotin], mAb, Mouse - 100 ug |
$335.00 |
|
A01490 |
THETM alpha Tubulin Antibody [HRP], mAb, Mouse - 100 ug |
$335.00 |
|
A01410-100 |
THETM alpha Tubulin Antibody, mAb, Mouse - 100 ug |
$225.00 |
|
A01203-40 |
β-Tubulin III Antibody, pAb, Rabbit - 40 ug |
$99.00 |
Y Shibuya, et al. Inhibiting ACAT1/SOAT1 in Microglia Stimulates Autophagy-Mediated Lysosomal Proteolysis and Increases Aβ1–42 Clearance. J Neurosci. 2014Qct.;34(43):14484-501
Liberman R., et al. Regulated Assembly of the V-ATPase is Increased During Cluster Disruption-Induced Maturation of Dendritic Cells Through a PI-3 Kinase/mTOR-dependent Pathway. J Biol Chem. 2014 Jan;289(3):1355-63.
Lakshmana MK., et al. Role of RanBP9 on amyloidogenic processing of APP and synaptic protein levels in the mouse brain. FASEB J. 2012 May;26(5):2072-83.
Schmid M., et al. Apicomplexan Parasite, Eimeria falciformis, Co-opts Host Tryptophan Catabolism for Life Cycle Progression in Mouse. J Biol Chem. 2012 Jun;287(24):20197-207.
Amita M., et al. Complete and unidirectional conversion of human embryonic stem cells to trophoblast by BMP4. Proc Natl Acad Sci U S A. 2013 Mar;110(13):E1212 - E1221.
Chen HW., et al. Inhibitory and combinatorial effect of diphyllin, a v-ATPase blocker, on influenza viruses. Antiviral Res. 2013 Sep;99(3):371-82.
Kuo HP., et al. Epigenetic Roles of MLL Oncoproteins Are Dependent on NF-κB. Cancer Cell. 2013 Oct;24(4):423-37
You can visit "Citations Database" for more citations.
Liberman R., et al. Regulated Assembly of the V-ATPase is Increased During Cluster Disruption-Induced Maturation of Dendritic Cells Through a PI-3 Kinase/mTOR-dependent Pathway. J Biol Chem. 2014 Jan;289(3):1355-63.
Lakshmana MK., et al. Role of RanBP9 on amyloidogenic processing of APP and synaptic protein levels in the mouse brain. FASEB J. 2012 May;26(5):2072-83.
Schmid M., et al. Apicomplexan Parasite, Eimeria falciformis, Co-opts Host Tryptophan Catabolism for Life Cycle Progression in Mouse. J Biol Chem. 2012 Jun;287(24):20197-207.
Amita M., et al. Complete and unidirectional conversion of human embryonic stem cells to trophoblast by BMP4. Proc Natl Acad Sci U S A. 2013 Mar;110(13):E1212 - E1221.
Chen HW., et al. Inhibitory and combinatorial effect of diphyllin, a v-ATPase blocker, on influenza viruses. Antiviral Res. 2013 Sep;99(3):371-82.
Kuo HP., et al. Epigenetic Roles of MLL Oncoproteins Are Dependent on NF-κB. Cancer Cell. 2013 Oct;24(4):423-37
You can visit "Citations Database" for more citations.
For more documents, please visit "Technical Support".
| Cat. No. A01410 | ||
|---|---|---|
Size |
Price |
|
| 100 ug | $225.00 | |
