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Resources » Research Applications » Stem Cell Research » Stem Cell Antibodies » Nestin Antibody, pAb, Rabbit

Nestin Antibody, pAb, Rabbit

Synonyms Rabbit Anti-Nestin pAb;
Description Nestin is a class IV intermediate filament protein which is abundantly expressed in neuroepithelial stem cells early in embryogenesis. It contains a short head domain and a long tail domain. Upon differentiation, nestin is down regulated and is replaced by other tissue specific intermediate filaments. It serves as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS.

GenScript Rabbit Anti-Nestin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from within residues 1280-1330 of human Nestin (Swiss Prot: P48681).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide from within residues 1280-1330 of human Nestin (Swiss Prot: P48681).
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-Nestin Polyclonal Antibody detects endogenous levels of human Nestin. It is predicted to react with mouse and rat according to sequence homology.
Species Reactivity Human. This product has not been tested with other species yet.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other Applications: user-optimized

  • Nestin Antibody, PAb, Rabbit
  • Nestin Antibody, PAb, Rabbit

    Western blot analysis of cell lysates using 1 µg/ml Rabbit Anti-Nestin Polyclonal Antibody (GenScript, A01413)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 220 KD
    Observed Size: 220 kD


Megan R. France., et al. Intraventricular injection of myxoma virus results in transient expression of viral protein in mouse brain ependymal and subventricular cells. J Gen Virol. 2011 Jan;92(1):195 - 199.

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Cat. No. A01413
Size
Price
40 ug $99.00