• English
  • Sign In
  • Contact Us
×
Catalog Products » Antibodies » Primary Antibodies » Control Antibodies » VDAC1 Antibody, pAb, Rabbit

VDAC1 Antibody, pAb, Rabbit

Synonyms Rabbit Anti-VDAC1 pAb;
Description Voltage dependent anion selective channel protein 1 (also known as VDAC1 or mitochondrial Porin) is the predominant protein in the outer mitochondrial membrane. VDAC1 is predicted to have a beta-barrel structure and consist of either 13 or 16 membrane spanning strands. In particular, VDAC1 can function as an NADH ferricyanide reductase. It plays a vital role in modulating endostatin induced endothelial cell apoptosis. Its activity is regulated by Nek1 (NIMA related protein kinase 1) through phosphorylation of VDAC1 protein on serine 193.

GenScript Rabbit Anti-VDAC1 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 150-200 of human VDAC1 (Swiss Prot: P21796).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide within residues 150-200 of human VDAC1 (Swiss Prot: P21796).
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-VDAC1 Polyclonal Antibody detects endogenous levels of human, mouse and rat VDAC1.
Species Reactivity Human, mouse, and rat. This product has not been tested with other species yet.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other Applications: user-optimized

  • VDAC1 Antibody, PAb, Rabbit
  • VDAC1 Antibody, PAb, Rabbit

    Western blot analysis of cell and tissue lysates using 1 µg/ml Rabbit Anti-VDAC1 Polyclonal Antibody (GenScript, A01419)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 31 KD
    Observed Size: 31 KD


Sayed D., et al. Transcriptional Regulation Patterns Revealed by High Resolution Chromatin Immunoprecipitation during Cardiac Hypertrophy. J Biol Chem. 2013 Jan;288(4):2546-58.

You can visit "Citations Database" for more citations.






For more documents, please visit "Technical Support".

Cat. No. A01419
Size
Price
40 ug $99.00