|Synonyms||Rabbit Anti-CHRNA7 pAb;|
|Description||CHRNA7 (Also known as Nicotinic Acetylcholine Receptor alpha 7) is a major component of brain nicotinic receptors that are blocked by alpha-bungarotoxin. The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 has a signal peptide, followed by an N-terminal extracellular domain, 3 membrane-spanning regions, an intracellular domain, a fourth transmembrane region, and an extracellular C-terminal tail. The nicotinic acetylcholine receptor alpha-7 subunit is required for acetylcholine inhibition of macrophage TNF release. The nicotinic acetylcholine receptor alpha-7 subunit is essential for inhibiting cytokine synthesis by the cholinergic antiinflammatory pathway. Upregulation of CHRNA7 receptors may be a compensatory response to maintain basocortical cholinergic activity during disease progression or may act with beta-amyloid in disease pathogenesis.
GenScript Rabbit Anti-CHRNA7 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal extracellular domain of human CHRNA7 (Swiss Prot: P36544).
|Immunogen||KLH-coupled synthetic peptide from N-terminal extracellular domain of human CHRNA7 (Swiss Prot: P36544).|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide|
|Reconstitution||Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.|
|Specificity||GenScript Rabbit Anti-CHRNA7 Polyclonal Antibody detects endogenous levels of human, mouse, and rat CHRNA7.
|Species Reactivity||Human, mouse, and rat. This product has not been tested with other species yet|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Other Applications: user-optimized
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