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THETM DYKDDDDK Tag Antibody [HRP], mAb, Mouse

Synonyms Anti Flag-tag mAb; Anti DYKDDDDK-tag mAb
Description GenScript THETM DYKDDDDK Tag Antibody [HRP], mAb, Mouse is HRP conjugated THETM Anti-DYKDDDDK Monoclonal Antibody (Mouse) (A00187) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of DYKDDDDK in fusion proteins.
Host Species Mouse
Conjugation Horseradish Peroxidase (HRP)
Immunogen A synthetic peptide (DYKDDDDK) coupled to KLH
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Purification Protein A affinity column

Subclass IgG2b, k
Clone ID 5A8E5
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.01% thimerosal
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity THETM DYKDDDDK Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

 THE<sup>TM</sup> DYKDDDDK Tag Antibody [HRP], MAb, Mouse
 THE<sup>TM</sup> DYKDDDDK Tag Antibody [HRP], MAb, Mouse

Western blot analysis of DYKDDDDK-tagged protein using THETM DYKDDDDK Tag Antibody [HRP], mAb, Mouse (GenScript, A01428, 1 µg/ml)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

Which resin yields 1 mg DYKDDDDK-tagged protein
per ml settled resin?

Find answer here...

GenScript's Anti-DYKDDDDK G1 resin

Cat. No.
Product Name
A00170-40 DYKDDDDK-tag Antibody, pAb, Rabbit
40 ug
A00187-100 THETM DYKDDDDK Tag Antibody, mAb, Mouse
100 ug
A01428-100 THETM DYKDDDDK Tag Antibody [HRP], mAb, Mouse
100 ug
A01632 THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse
100 ug
L00432-25 Anti-DYKDDDDK G1 Affinity Resin
25 ml
L00432-10 Anti-DYKDDDDK G1 Affinity Resin
10 ml
L00455C DYKDDDDK Tag Antibody Plate (Clear, 8X12 strip)
5 plates
A01811-100 THETM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse
100 ug
Fan Y, et al. Profilin-1 phosphorylation directs angiocrine expression and glioblastoma progression through HIF-1α accumulation. Nat Cell Biol. 2014May;16(5):445-56

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Cat. No. A01428
100 ug $149.00
24 hour