Catalog Products » Antibodies » THE™ Elite Antibodies » THETM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse

THETM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse

Synonyms anti Flag-tag mAb; anti DYKDDDDK-tag mAb
Description GenScript THETM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse is the biotin conjugated THETM DYKDDDDK Tag Antibody, mAb, Mouse (A00187) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of DYKDDDDK in fusion proteins.
Host Species Mouse
Conjugation Biotin
Immunogen A synthetic peptide (DYKDDDDK) coupled to KLH
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A affinity column

Subclass IgG2b, k
Clone ID 5A8E5
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity THETM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

 THE<sup>TM</sup> DYKDDDDK Tag Antibody [Biotin], MAb, Mouse
 THE<sup>TM</sup> DYKDDDDK Tag Antibody [Biotin], MAb, Mouse

Loading: N-terminal DYKDDDDK-fusion protein (400 ng, 100 ng, 25 ng)
Primary antibody: 1 μg/ml THETM Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Biotin) (GenScript, Cat. No. A01429, Lot No. A213902)
Secondary antibody: Streptavidin-HRP conjugate (GenScript, 1:5,000, M00091)

Fan Y, et al. Profilin-1 phosphorylation directs angiocrine expression and glioblastoma progression through HIF-1α accumulation. Nat Cell Biol. 2014May;16(5):445-56

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Cat. No. A01429
100 ug $149.00
24 hour