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Catalog Products » Antibodies » Epitope Tag Antibodies » THETM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse

THETM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse

Synonyms anti Flag-tag mAb; anti DYKDDDDK-tag mAb
Description GenScript THETM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse is the biotin conjugated THETM DYKDDDDK Tag Antibody, mAb, Mouse (A00187) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of DYKDDDDK in fusion proteins.
Host Species Mouse
Conjugation Biotin
Immunogen A synthetic peptide (DYKDDDDK) coupled to KLH
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A affinity column

Subclass IgG2b, k
Clone ID 5A8E5
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity THETM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

  •  THE<sup>TM</sup> DYKDDDDK Tag Antibody [Biotin], MAb, Mouse
  •  THE<sup>TM</sup> DYKDDDDK Tag Antibody [Biotin], MAb, Mouse

    Loading: N-terminal DYKDDDDK-fusion protein (400 ng, 100 ng, 25 ng)
    Primary antibody: 1 μg/ml THETM Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Biotin) (GenScript, Cat. No. A01429, Lot No. A213902)
    Secondary antibody: Streptavidin-HRP conjugate (GenScript, 1:5,000, M00091)


Cat. No.
Product Name
Price
A00170-40
DYKDDDDK-tag Antibody, pAb, Rabbit - 40 ug
$99.00
A00187-100
THETM DYKDDDDK Tag Antibody, mAb, Mouse - 100 ug
$139.00
A00187-200
THETM DYKDDDDK Tag Antibody, mAb, Mouse - 200 ug
$256.00
A01428-100
THETM DYKDDDDK Tag Antibody [HRP], mAb, Mouse - 100 ug
$149.00
A01632
THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse - 100 ug
$149.00
L00425
Anti-DYKDDDDK IP Resin - 1 ml
$199.00
L00432-1
Anti-DYKDDDDK G1 Affinity Resin - 1 ml
$281.00
L00432-25
Anti-DYKDDDDK G1 Affinity Resin - 25 ml
$2591.00
L00432-5
Anti-DYKDDDDK G1 Affinity Resin - 5 ml
$663.00
L00455C
DYKDDDDK Tag Antibody Plate (Clear, 8X12 strip) - 5 plates
$229.00
L00455W
DYKDDDDK Tag Antibody Plate (White, 96-well) - 5 plates
$229.00
L00455B
DYKDDDDK Tag Antibody Plate (Black, 96-well) - 5 plates
$229.00
A01809-100
THETM DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse - 100 ug
$167.00
A01810-100
THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse - 100 ug
$167.00
A01811-100
THETM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse - 100 ug
$167.00
 
Fan Y, et al. Profilin-1 phosphorylation directs angiocrine expression and glioblastoma progression through HIF-1α accumulation. Nat Cell Biol. 2014May;16(5):445-56

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Cat. No. A01429
Size
Price
100 ug $149.00
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