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Catalog Products » Antibodies » Primary Antibodies » Cell Marker Antibodies » PDI Antibody, pAb, Rabbit

PDI Antibody, pAb, Rabbit

PDI Antibody, PAb, Rabbit

Western blot analysis of cell and tissue lysates using 1 µg/ml Rabbit Anti-PDI Polyclonal Antibody (GenScript, A01434)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 57 KD
Observed Size: 60 KD

PDI Antibody, PAb, Rabbit

Flow cytometric analysis of HepG2 cells using PDI Antibody, pAb, Rabbit (GenScript, A01434; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

Synonyms PDI A1 antibody; ER Marker
Description Protein Disulphide Isomerase (PDI) is a microsomal enzyme which locates in endoplasmic reticulum lumen. The enzyme catalyzes the formation and isomerization of intermolecular disulphide-bond that stabilizes three dimensional structure of many extracellular proteins. The upregulation of PDI represents an adaptive response to the accumulation of immature and denatured proteins which cause ER dysfunction in neurodegenerative disorders.
GenScript Rabbit Anti-PDI Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 100-150 of human PDI (Swiss Prot: P07237).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide within residues 100-150 of human PDI (Swiss Prot: P07237).
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-PDI Polyclonal Antibody detects endogenous levels of human, mouse, and rat PDI.
  • Positive Control:
  • K562, HepG2, A431, Hela, Ramos, mouse brain and rat brain lysates
    Species Reactivity Human, mouse, and rat. This product has not been tested with other species yet.
    Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.05-0.2 µg/ml
    Western blot: 0.5-1 µg/ml
    Flow cytometry: 1-3 µg for 1 x 106 cells
    Other Applications: user-optimized

    • PDI Antibody, PAb, Rabbit
    • PDI Antibody, PAb, Rabbit

      Western blot analysis of cell and tissue lysates using 1 µg/ml Rabbit Anti-PDI Polyclonal Antibody (GenScript, A01434)
      The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
      Predicted Size: 57 KD
      Observed Size: 60 KD

    • PDI Antibody, PAb, Rabbit
    • PDI Antibody, PAb, Rabbit

      Flow cytometric analysis of HepG2 cells using PDI Antibody, pAb, Rabbit (GenScript, A01434; shaded histogram)
      or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.




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    Cat. No. A01434
    Size
    Price
    40 ug $99.00