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Home » Gene Synthesis & Molecular Biology Services » NSG1 Antibody, pAb, Rabbit

NSG1 Antibody, pAb, Rabbit

NSG1 Antibody, PAb, Rabbit

Western blot analysis of tissue lysates using 1 µg/ml Rabbit Anti-NSG1 Polyclonal Antibody (GenScript, A01442)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 21 KD
Observed Size: 21 KD

NSG1 Antibody, PAb, Rabbit

NSG1 Antibody, PAb, Rabbit

Flow cytometric analysis of Ramos cells using NSG1 Antibody, pAb, Rabbit (GenScript, A01442; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

NSG1 Antibody, PAb, Rabbit

ImmunohistochemistryanalysisofhumanBrain, Cerebellum tissue slide (Paraffin embedded) using NSG1 Antibody, pAb, Rabbit (GenScript, Cat. No.A01442, 10 ug/ml).

Synonyms Rabbit Anti-NSG1 Polyclonal Antibody;
Description NSG1, also known as Neuron-specific protein family member 1, contains an N-terminal nuclear localization motif and a central hydrophobic segment. NSG1 localizes to the nucleus in mitotic neuronal cell cultures and to the cytoplasm in postmitotic neurons. Mouse and human NSG1 share 97% sequence identity and they differs from each other by only 5 amino acid residues. The protein is expressed in all regions of rat brain. In hamster, it was preferentially expressed in adult brain.
GenScript Rabbit Anti-NSG1 Polyclonal Antibody is developed in rabbit using purified His-tagged human NSG1 (Swiss Prot: P42857) full length recombinant protein.
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Purified His-tagged human NSG1 (Swiss Prot: P42857) full length recombinant protein.
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-NSG1 Polyclonal Antibody detects endogenous levels of human, mouse, and rat NSG1.
Positive Control: mouse brain and rat brain.
Species Reactivity Human, mouse, and rat. This product has not been tested for other species yet.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunohistochemistry:5-10 µg/ml
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Immunoprecipitation (IP): 2-10 µg/mg of lysate
Flow cytometry: 1-3 µg for 1 x 106 cells
Other Applications: user-optimized

  • NSG1 Antibody, PAb, Rabbit
  • NSG1 Antibody, PAb, Rabbit

    Western blot analysis of tissue lysates using 1 µg/ml Rabbit Anti-NSG1 Polyclonal Antibody (GenScript, A01442)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 21 KD
    Observed Size: 21 KD

  • NSG1 Antibody, PAb, Rabbit
  • NSG1 Antibody, PAb, Rabbit

  • NSG1 Antibody, PAb, Rabbit
  • NSG1 Antibody, PAb, Rabbit

    Flow cytometric analysis of Ramos cells using NSG1 Antibody, pAb, Rabbit (GenScript, A01442; shaded histogram)
    or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

  • NSG1 Antibody, PAb, Rabbit
  • NSG1 Antibody, PAb, Rabbit

    ImmunohistochemistryanalysisofhumanBrain, Cerebellum tissue slide (Paraffin embedded) using NSG1 Antibody, pAb, Rabbit (GenScript, Cat. No.A01442, 10 ug/ml).




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Cat. No. A01442
Size
Price
40 ug $99.00