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Catalog Products » Antibodies » Epitope Tag Antibodies » KLH Antibody, mAb, Mouse

KLH Antibody, mAb, Mouse

Synonyms Keyhole Limpet Hemocyanin mAb ; KLH
Description Keyhole limpet hemocyanin (KLH) is a high molecular-weight and copper-containing protein. It is found in the hemolymph of sea mollusk Megathura crenulata. The KLH protein is potently immunogenic yet safe in human and is therefore extensively used as a carrier protein in the production of antibodies for research as well as in other biotechnology and therapeutic applications.
Host Species Mouse
Conjugation Unconjugated
Immunogen Full keyhole limpet hemocyanin
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Purification Protein A affinity column

Subclass Mouse IgG1,κ
Clone ID 3C1G5
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity No cross-reactivity with BSA and OVA are detected
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Dot blot: 0.1-1.0 µg/ml
Other: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

KLH Antibody, MAb, Mouse
KLH Antibody, MAb, Mouse

Primary antibody:
1 μg/ml Mouse Anti-KLH Monoclonal Antibody (GenScript, Cat. No. A01486, Lot No. A207906)
Secondary antibody:
IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG (ROCKLAND, 1:10,000, 610-132-121)


Tawk L., et al. A key role for Plasmodium subtilisin-like SUB1 in egress of malaria parasites from host hepatocytes. J Biol Chem. 2013 Nov;288(46):33336-46.

Ling Xiao., et al. KIBRA Regulates Hippo Signaling Activity via Interactions with Large Tumor Suppressor Kinases. J Biol Chem. 2011 Mar;286(10):7788 - 7796.

Ahrman E, et al. Novel COMP Neoepitopes Identified in Synovial Fluids from Patients with Joint Diseases using Affinity Chromatography and Mass Spectrometry. J Biol Chem. 2014June;

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Cat. No. A01486
Size
Price
40 ug $99.00
24 hour
 
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