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Catalog Products » Antibodies » Primary Antibodies » Cell Marker Antibodies » Cytochrome C Antibody, pAb, Rabbit

Cytochrome C Antibody, pAb, Rabbit

Cytochrome C Antibody, PAb, Rabbit

Western blot analysis of cell lysates using Rabbit Anti-Cytochrome C Polyclonal Antibody (GenScript, A01507, 2 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 12 KD Observed Size: 12 KD

Cytochrome C Antibody, PAb, Rabbit

Flow cytometric analysis of Ramos cells using Cytochrome C Antibody, pAb, Rabbit (GenScript, A01507; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

Synonyms Rabbit Anti-Cytochrome C polyclonal antibody; CYC antibody
Description Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome C has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome C is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis. GenScript Rabbit Anti-Cytochrome C Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human Cytochrome C (Swiss Prot: P99999).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide from N-terminal of human Cytochrome C (Swiss Prot: P99999).
Purification Immunoaffinity chromatography.

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-Cytochrome C Polyclonal Antibody detects endogenous levels of human and mouse Cytochrome C. Predicted to react with rat Cytochrome C according to sequence homology.
Species Reactivity Human and mouse.
Predicted Band Size 12 KD
Observed Band Size 12 KD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1-2 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user-optimized

  • Cytochrome C Antibody, PAb, Rabbit
  • Cytochrome C Antibody, PAb, Rabbit

    Western blot analysis of cell lysates using Rabbit Anti-Cytochrome C Polyclonal Antibody (GenScript, A01507, 2 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 12 KD Observed Size: 12 KD

  • Cytochrome C Antibody, PAb, Rabbit
  • Cytochrome C Antibody, PAb, Rabbit

    Flow cytometric analysis of Ramos cells using Cytochrome C Antibody, pAb, Rabbit (GenScript, A01507; shaded histogram)
    or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.


Li B., et al. Titanium Dioxide Nanoparticles Relieve Silk Gland Damage and Increase Cocooning of Bombyx Mori under Phoxim-Induced Toxicity. J Agric Food Chem. 2013 Dec;61(50):12238-43.

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Cat. No. A01507
Size
Price
40 ug $99.00