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Home » Gene Synthesis & Molecular Biology Services » NQO1 antibody, pAb, Rabbit

NQO1 antibody, pAb, Rabbit

NQO1 Antibody, PAb, Rabbit

Western blot analysis of cell lysates using Rabbit Anti-NQO1 Polyclonal Antibody (GenScript, A01511, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 31 KD Observed Size: 31 KD

NQO1 Antibody, PAb, Rabbit

Flow cytometric analysis of A549 cells using NQO1 antibody, pAb, Rabbit (GenScript, A01511; shade histogram) or with an isotype control
antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

Synonyms Rabbit Anti-NQO1 polyclonal antibody; NQO1 antibody
Description Quinone oxidoreductase 1 (NQO1) is a cytosolic flavoenzyme that catalyzes the two-electron reduction of a broad range of substrates. It is one member of the NAD(P)H dehydrogenase (quinone) family. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinones thus preventing the formation of reactive oxygen species. NQO1 can serve as a detoxification enzyme for it has the ability to reduce quinone substrates directly to their less toxic hydroquinone derivatives. NQO1 can also function as an antioxidant enzyme reducing ubiquinone and vitamin E quinone to their antioxidant forms. GenScript Rabbit Anti-NQO1 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human NQO1 (Swiss Prot: P15559).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide from C-terminal of human NQO1 (Swiss Prot: P15559).
Purification Immunoaffinity chromatography.

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-NQO1 Polyclonal Antibody detects endogenous levels of human NQO1.
Species Reactivity Human
Predicted Band Size 31 KD
Observed Band Size 31 KD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other Applications: user optimized

  • NQO1 Antibody, PAb, Rabbit
  • NQO1 Antibody, PAb, Rabbit

    Western blot analysis of cell lysates using Rabbit Anti-NQO1 Polyclonal Antibody (GenScript, A01511, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 31 KD Observed Size: 31 KD

  • NQO1 Antibody, PAb, Rabbit
  • NQO1 Antibody, PAb, Rabbit

    Flow cytometric analysis of A549 cells using NQO1 antibody, pAb, Rabbit (GenScript, A01511; shade histogram) or with an isotype control
    antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.




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Cat. No. A01511
Size
Price
40 ug $99.00