|Description||PTF1A is a basic helix-loop-helix transcription factor that contains one basic helix-loop-helix (bHLH) domain. PTF1A interacts with RBP-L protein and a common type I bHLH protein to form the pancreas transcription factor (PTF1). It is present in the dorsal neural tube of early stage embryos, an embryonic structure that gives rise to the dorsal horn of the spinal cord in the mature animal. PTF1A plays a fundamental role in exocrine and endocrine pancreas development in mice.
GenScript PTF1A Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 280–330 of human PTF1A (Swiss Prot: Q7RTS3).
|Immunogen||KLH-coupled synthetic peptide within residues 280-330 of human PTF1A (Swiss Prot: Q7RTS3).|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide|
|Reconstitution||Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.|
|Specificity||GenScript PTF1A Antibody detects endogenous levels of human, mouse and rat PTF1A.
Positive Control: Mouse brain
|Species Reactivity||Human, mouse and rat|
|Predicted Band Size||35 KD|
|Observed Band Size||42 KD|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1-2 µg/ml.
Other Applications: user optimized.
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)
Western blot analysis of cell and tissue lysates using PTF1A Antibody (GenScript, A01521, 1 µg/ml)
Lane 1: E.coli lysate expressing human PTF1A
Lane 2: Mouse brain tissue lysate.
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 35 KD
Observed Size: 42 KD