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Home » Gene Synthesis & Molecular Biology Services » BiP Antibody, pAb, Rabbit

BiP Antibody, pAb, Rabbit

BiP Antibody, PAb, Rabbit

Western blot analysis of tissue lysates using BiP Antibody (GenScript, A01522, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 78 KD
Observed Size: 78 KD

BiP Antibody, PAb, Rabbit

Western blot analysis of cell lysates using BiP Antibody (GenScript, A01522, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 78 KD
Observed Size: 78 KD and 50 KD

BiP Antibody, PAb, Rabbit

Flow cytometric analysis of A549 cells using BiP Antibody, pAb, Rabbit (GenScript, A01522; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

Synonyms GRP78 BiP Antibody; HSPA5 Antibody
Description BiP, also known as GRP78 BiP, is one member of the family of 70KD heat shock proteins. It may associate transiently with a variety of newly synthesized secretory and membrane proteins. And it binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding. The Lys-Asp-Glu-Leu (KDEL) sequence of BiP is present at the C terminus of BiP and this sequence seems to be necessary for retention.
GenScript BiP Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 250–300 of human BiP (Swiss Prot: P11021).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide within residues 250-300 of human BiP (Swiss Prot: P11021).
Purification Immunoaffinity chromatography.

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript BiP Antibody detects endogenous levels of human and mouse BiP. It is predicted to react with rat BiP according to sequence homology.
  • Positive Control: A431, Mouse brain and Mouse liver
  • Species Reactivity Human and mouse
    Predicted Band Size 78 KD
    Observed Band Size 78 KD
    Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product
    Western blot: 0.5-1 µg/ml
    Flow cytometry: 1-3 µg for 1 x 106 cells
    Other Applications: user-optimized

    • BiP Antibody, PAb, Rabbit
    • BiP Antibody, PAb, Rabbit

      Western blot analysis of tissue lysates using BiP Antibody (GenScript, A01522, 1 µg/ml)
      The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
      Predicted Size: 78 KD
      Observed Size: 78 KD

    • BiP Antibody, PAb, Rabbit
    • BiP Antibody, PAb, Rabbit

      Western blot analysis of cell lysates using BiP Antibody (GenScript, A01522, 1 µg/ml)
      The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
      Predicted Size: 78 KD
      Observed Size: 78 KD and 50 KD

    • BiP Antibody, PAb, Rabbit
    • BiP Antibody, PAb, Rabbit

      Flow cytometric analysis of A549 cells using BiP Antibody, pAb, Rabbit (GenScript, A01522; shaded histogram)
      or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.




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    Cat. No. A01522
    Size
    Price
    40 ug $99.00